Faim2 (NM_001038658) Mouse Untagged Clone

CAT#: MC211327

Faim2 (untagged) - Mouse Fas apoptotic inhibitory molecule 2 (Faim2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Faim2
• Synonyms: 2900002L20Rik; Lfg; Lfg2; NMP25; Nmp35; Tmbim2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211327 representing NM_001038658
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAGGGAAAGCTCTCTGTGGCTAACAAGGCCCCCGGGACTGAGGGGCAGCAGCACCAGGCAAATG
  GAGAGAAGAAGGATGCTCCGGCAGTGCCCTCGGCGCCACCCTCCTATGAGGAGGCCACCTCTGGAGAGGG
  GCTGAAGGCAGGGACCTTCCCCCAGGGCCCAACAGCTGTGCCACTCCATCCAAGCTGGGCCTACGTGGAC
  CCCAGACATCACGAGCATTTCACCACCTTCAGCTGGGATGACCAGAAAGTTCGTCGGCTCTTCATCAGAA
  AGGTCTATACCATCCTATTGGTCCAGCTGCTGGTGACCTTGGCTGTGGTGGCTCTCTTTACCTTCTGTGA
  TGTCGTTAAGGACTATGTCCAGGCTAACCCCGGCTGGTACTGGGCGTCCTATGCCGTGTTCTTTGCAACT
  TACCTGACTCTGGCTTGCTGTTCTGGACCCAGGAGACATTTCCCTTGGAACCTGATTCTGCTGACCATCT
  TTACCCTGTCCATGGCCTACCTCACTGGGATGCTGTCCAGTTACTACAACACCACATCTGTGCTGCTGTG
  CCTGGTCATCACGGCTCTCGTCTGCCTCTCGGTCACCATCTTCAGTTTCCAGACCAAGTTTGACTTCACC
  TCCTGTCAGGGTGTGCTGTTTGTGCTGCTCATGACCCTCTTCTTCAGTGGACTCCTCCTCGCTGTCCTCC
  TGCCCTTCCAATACGTCCCCTGGCTCCACGCGGTCTATGCTGTACTGGGAGCGGGTGTGTTCACGTTGTT
  CCTGGCCTTTGATACCCAGTTGCTGATGGGTAACCGTCGACACTCGCTGAGCCCCGAGGAGTATATTTTT
  GGAGCCCTCAACATCTACTTAGACATTATCTATATCTTCACCTTCTTCCTGCAGCTTTTTGGCACCAACC
  GGGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038658
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038658.2, NP_001033747.1
• RefSeq Size: 4632 bp
• RefSeq ORF: 918 bp
• Locus ID: 72393
• UniProt ID: Q8K097
• Cytogenetics: 15 F1
• Gene Summary: Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. Plays a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.