Rfc5 (NM_028128) Mouse Untagged Clone

CAT#: MC211296

Rfc5 (untagged) - Mouse replication factor C (activator 1) 5 (Rfc5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rfc5
• Synonyms: 36.5kDa; 36kDa; 2610020K06Rik; 2610209F07Rik; Recc5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211296 representing NM_028128
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGCCGCGCCTTCGCAGCAGCGGCCCGCGGCGGCCCGGGCCCGGAACCTGCCCTGGGTTGAGA
  AGTACCGGCCACAGACCCTGGCCGATCTCATTTCTCACCAGGACATTCTGAGTACCATTCAGAAGTTCAT
  CAGTGAAGACCGCCTGCCACACCTACTTCTCTATGGCCCTCCAGGGACAGGAAAGACATCCACCATCCTG
  GCCTGTGCCAAGCAACTGTACAAAGATAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCTGACG
  ACCGAGGGATCGATATTGTTCGGGGGCCAATCCTCAGCTTTGCCAGCACAAGGACAATCTTCAAGAAAGG
  GTTTAAGCTTGTGATCCTGGACGAAGCCGACGCCATGACTCAGGATGCCCAGAACGCCTTGAGACGAGTG
  ATTGAGAAGTTCACAGAAAACACCAGGTTCTGCCTCATCTGTAACTACCTGTCCAAGATCATCCCTGCCT
  TGCAGTCTCGGTGCACGAGGTTCCGATTTGGCCCTCTGACACCCGAGCTCATGGTCCCTCGACTGGAACA
  TGTAGTACAAGAAGAGAATGTGGATATAAGTGAAGATGGAATGAAGGCCCTTGTCACTCTGTCCAGTGGG
  GACATGCGAAGGGCTTTGAACATCCTTCAGAGTACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTG
  TCTACACCTGCACCGGACACCCACTCAAGACGGACATTGCCAATATACTGGACTGGATGCTCAATCAGGA
  CTTCACCACTGCCTACAAAAACATCATGGAGCTGAAGACTCTGAAGGGCTTGGCGCTGCATGACATCCTG
  ACAGAGGTTCACTTGTTTGTACACAGAGTTGACTTTCCATCTTCAGTTCGGATACACTTATTGACCAAAA
  TGGCAGACATCGAGTACAGACTTTCTGTTGGCACCAGTGAGAAGATACAGCTGAGCTCCCTCATCGCCGC
  TTTTCAAGTCACCAGAGACCTGATAGTTGCAGAGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028128
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028128.1, NP_082404.1
• RefSeq Size: 1750 bp
• RefSeq ORF: 1020 bp
• Locus ID: 72151
• UniProt ID: Q9D0F6
• Cytogenetics: 5 F
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.[UniProtKB/Swiss-Prot Function]