Pygo1 (NM_028116) Mouse Untagged Clone

CAT#: MC211293

Pygo1 (untagged) - Mouse pygopus 1 (Pygo1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pygo1
• Synonyms: 2600014C22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211293 representing NM_028116
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCGGAACAGGACAAGGAGCCCATCGCGCTGAAGAGAGTTAGAGGTGGTGACAGTGGACTGGATG
  GGTTAGGAGGGCCCAATATACAACTCGGAAGCCCAGATAAGAAAAAACGCAAGGCCAACACACAGGGATC
  TTCCTTTCCTCCGCTGTCAGAGTACGCGCCACCACCGAATCCAAACTCCGACCATCTAGTGGCTGCCAAT
  CCGTTCGATGACAGCTACAACACCATTTCCTATAAACCACTGCCTTCATCTAATCCATATCTTGGCCCTG
  GGTATCCTGGCTTTGGAGGCTACAGCACATTCAGAATGCCACCCCACGTCCCTCCAAGAATGTCTTCTCC
  CTACTGTGGTCCTTACTCACTCAGGAATCAGCCCCACCCATTTCCTCAGAATCCGTTGGGCATGGGCTTT
  AACCGGCCTCATGCTTTTAACTTTGGGCCGCATGATAATTCGAATTTTGGAAACCCACCTTATAATAATG
  TACTGACTCAGGACATTAACATGCCCGGTCAGCATTTTAGACAAGGCTCTGCTGAAAACTTCAGTCAGAT
  TCCCCCGCAGAATGTTGGCCAAGTGTCTAACCCTGACCTCGCATCTAATTTTGCCCCTGGGAATAATTCA
  AATTTTACCTCTCCGTTAGAAACGAATCATTCGTTTATTCCACCCCCAAACGCGTTTGGCCAAGCAAAAG
  CTCCACTTCCCAAACAAGACTTCACTCAAGGGGCAACCAAAACCCCGAATCAGAATTCGTCCACTCACCC
  ACCTCACCTAAATATGGAGGATCCAGTCAATCAGAGTAACGTCGAGTTAAAAAATGTCAACAGAAACAAC
  GTTGTCCAAGAGAACAGCCGTTCGGGCAGCGCAGAGGCCACCAACAACCATGCGAATGGGACCCAGAACA
  AGCCCCGGCAGCCCAGGGGCGCAGCTGACCTGTGCACCCCCGACAAAAGCCGCAAGTTCTCCCTGCTCCC
  CAGCCGGCATGGCCATTCCTCCTCTGACCCTGTGTACCCGTGCGGGATTTGTACAAATGAAGTGAATGAC
  GATCAGGACGCCATTCTGTGTGAAGCCTCTTGTCAGAAGTGGTTTCATCGCATCTGCACTGGAATGACCG
  AAACAGCCTACGGGCTCCTGACAGCGGAAGCATCCGCAGTGTGGGGCTGTGACACGTGCATGGCTGACAA
  GGATGTCCAGCTCATGCGCACTAGAGAGGCCTTTGGTCCACCTGCCGTGGGCGGCGATGCCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_028116
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028116.2, NP_082392.1
• RefSeq Size: 1745 bp
• RefSeq ORF: 1254 bp
• Locus ID: 72135
• UniProt ID: Q9D0P5
• Cytogenetics: 9 D
• Gene Summary: Involved in signal transduction through the Wnt pathway.[UniProtKB/Swiss-Prot Function]