Trub1 (NM_028115) Mouse Untagged Clone

CAT#: MC211291

Trub1 (untagged) - Mouse TruB pseudouridine (psi) synthase homolog 1 (E. coli) (Trub1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trub1
• Synonyms: 2610009I02Rik; 9030425C13Rik; AI448235; BB118943; C76870
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211291 representing NM_028115
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGCAGAGGCAGAGGTGGTTTCACCTCTGATTGTGGACACAGCTCCTGACACTTCAGGAACAG
  CCGAGGCCTCTGTAGCAGCATCGGTGGCGGAAGCGGCCAGGACTGAATCGCAAGCCCCGGCCTCCAAGGC
  CGCCCTGGCTGCTAAGCTCATGTCGCTGAGCGGCGTGTTCGCCGTGCACAAGCCCAAAGGGCCCACTTCA
  GCCGAGCTGCTGAACCGGCTGAAGGAGAAGCTGCTGGCAGAAGCTGGAATGCCTTCTCCAGAATGGAACA
  AGAGGCAAAAGCAGACATTGAAAGTCGGACACGGAGGGACGCTGGACAGTGCAGCGCAAGGTGTTCTGGT
  GGTTGGGATTGGAAGAGGAACAAAAATGTTGACCAGTATGTTGTCGGGGTCCAAGAGGTATATCACCATC
  GGAGAACTGGGGAAAGCCACGGACACCCTGGATTCTACTGGGAAGGTAACGGAAGAGAAGCCGTATGATA
  AAATAACACGAGAAGATATCGAAGGCATTCTGCAGAAGTTTACTGGGAATATAATGCAAGTACCCCCGCT
  CTATTCTGCGCTGAAGAAGGATGGGCAGAGACTCTCAACGCTAATGAAGAAAGGCAAGGTGGTGGAGGCC
  AGACCGGCCCGGCCCGTGACTGTCCACAGTATCTCCCTCCTGAAGTTCCAGCCTCCGTTCTTCACGCTGG
  ATGTTGAATGCGGAGGAGGCTTTTACATCCGGAGCCTGGTCAGTGACATCGGCAAAGAGCTCTCTTCCTG
  TGCCAGCGTGCTGGAGCTGACCCGCACCAAACAGGGGCCCTTCACACTTGCACAGCACGCCCTCCCCGAA
  GACAGGTGGACCATCGATGACATCGAACAGTCCCTCGAGCGCTGCACGTCTCTTCTGCCGGAGGAGCTGA
  CATTTAAAAAACTAAAATCTGACAACTCCAGTGACCAGGTTATAAGCTGTGGATATATAACTCTCAGGGA
  CACAAAGAGAGAAGATGACGCAGTTAAGACACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028115
• Insert Size: 1017 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028115.3, NP_082391.1
• RefSeq Size: 3795 bp
• RefSeq ORF: 1017 bp
• Locus ID: 72133
• UniProt ID: Q8C0D0
• Cytogenetics: 19 D2
• Gene Summary: Pseudouridine synthase that catalyzes pseudouridylation of mRNAs. Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure. Constitutes the major pseudouridine synthase acting on mRNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.