Rnf135 (NM_028019) Mouse Untagged Clone

CAT#: MC211263

Rnf135 (untagged) - Mouse ring finger protein 135 (Rnf135), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf135
• Synonyms: 0610037N03Rik; 2410006N06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211263 representing NM_028019
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTTTGTTCTGGCAATGCAGTCCCCGTGTGGCTGAGCGAGGACGACCTGAGCTGCATTATCT
  GCCAAGGGCTACTAGACCAGCCCACCACGCTGCCCTGTGGCCACAGCTTCTGTCTCCGGTGCCTCCATGA
  CCTGTGGGTCTCGAAGCGTGGGGCCGTGGATGGCTGCCCTTGGGCTTGCCCCATATGTCGGAAGGGTCCC
  TTGACAAAGCCCAAGCTGCACAAGAACCCGCTGCTGCAAGACCTGGTGGACAAGTACCTCCAAGCGGCGC
  GGGAGGTCGAGGCTGGCTCGGAGCCTGAACCCGCCCCCGCGCCGCGTTCTGCGCCCCAGGTGACAGTACA
  GAAAAGTACCACAAACGTTATCCAGGAGCTGACTGACATGGTGAGACAGCTTGTAGACGATGTCAAGAGC
  CTTCAAACGCAAAGACCCAACTTGGGATCTGGACAGGACAATGCACAAGGCACCCCACCCACGGATTCTT
  CCTCTGAGGGGGAACATTCCTTGGATTCTCCAAAGCTGGTGACCTTCAGTATATCTCAGAAGAAAATTCA
  GGAGATTCTCCATAACCTGGAAGAAATTCAGGAGAAACTGCAAGGGAGTGTCCCAGGGAGAGCGCCCCCT
  AGAGAGCGAGTTCAGGAAATGACATCTTCCTTGTGCCTGCTGCCTGACCAGAGGCGTCCTGCACCCAGGA
  AAGCTTCTCACTTGTCCCTGTGGGCCATCAGTCCAACCTTTGACCTGAGGACCCTCTCCTATAACCTGGA
  GGTTTCCAACAATAGCCGGAGAGTGACTGTGTCTCGTGGTGATCTGCACACCTATCACTGGAGTCCCCAG
  AGATTTTCAATTAGCCAGGTCTTTTGTTCTCAGGCCCTCTCTTCTGGACAGAAGTACTGGGAAGTGGACA
  CTAGGAACTGTAGCCACTGGGCCATCGGGGTAGCTTCTTGGGGCATGAAGCGGGACGGGATGCTGGGAAG
  GACTATGGACTCCTGGTGTATAGAGTGGAGAGGGCCTGGCCAGTTCTCTGCTTGGGCCAAGATGAAGAAA
  ACAGACTTGCAGTCGGACCTCCCTGAGGTTGTGGGCGTGTGGCTGGACCTGGAGTCGGGGGAGCTTGCCT
  TCTACGCAGTGGCCGATCACGAGAGGCTCCTGTATGAGTGTGAGGTCTCTAGCTCCTCTCCTCTGCATCC
  CGCCTTCTGGCTGTATGGCTTAAGTCCTGGAAACTACCTGGAAATAAAGCAGCTAAACACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028019
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028019.3, NP_082295.1
• RefSeq Size: 1959 bp
• RefSeq ORF: 1254 bp
• Locus ID: 71956
• UniProt ID: Q9CWS1
• Cytogenetics: 11 47.59 cM
• Gene Summary: Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.[UniProtKB/Swiss-Prot Function]