Lrrc34 (NM_027941) Mouse Untagged Clone

CAT#: MC211226

Lrrc34 (untagged) - Mouse leucine rich repeat containing 34 (Lrrc34), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrrc34
• Synonyms: 1700007J06Rik; SPATA34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211226 representing NM_027941
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTCCAGGCCCAGTACTGTTACTTATGTTCAGAAAACATACGGAAAACTAATCCTTTCATAC
  TGAATATACTCCAAAAACTGGATGAAGAAATTGAAAAGCGACCCAAAGAAAAATTTACCGTAAATATTGC
  TGGTAACAATCGCTTAGACTCCGGACAAAGAATAACAGGGGAAGACTTTTGGCTTCTTTCCAAAACTTTA
  AGGAACCAGCCATGTATCAGTGGTGTGGATGTGAGGTATAACCTCATCGGCGATGTCGGTGCCTTCTATG
  CTGCGAAACTACTTCAGAAACAACCAAGTATCACTTATTTAAACCTTATGTTTAATGATATTGGACCTGA
  AGGTGGAGAATTGATTGCTAAAGCACTACATAAAAACAAAACTCTTAAGTATTTGAGAATGACGGGAAAT
  AAGATTGAGAACACAGGAGGCATGCTTTTTGCTGCAATGCTGCAGATGAACTCAAGCCTGGAGAAACTGG
  ATCTGGGCGACTGTGACCTGGGACTGCAATGTGTGATCGCATTTTCTACGGTACTAACTCAAAACCAAGC
  AATCAAGGGAATAAACCTGAACCGGCCTATACTGTACGGAGAACAGGAAGAGTCTACTGTCCACATTGGC
  CACATGTTGAAAGAAAATCATGTCCTTGTTGAGCTACATATGTGCAAACATGGCATGAAAAATTATGGTT
  TACAGCAGTTATGCAATGCACTGTATTTGAACAGTAGCCTACGCTACCTTGATGTCAGCTGCAATAAAAT
  AACTCGTGATGGAATGGTGTTTTTGGCTGATGTCCTAAAAAGCAATACTACTCTGGAAGTACTAGATCTT
  TCTTTTAACAGAATAGAAACTGCAGGCGCAAAGTACCTCAGTGAAACTCTAACTTCCCACAACAGGAGTC
  TTAAAGCATTGTCTGTAGTCAGCAACAAGATAGAAGGAGAGGGGCTTGTTGCACTTTCCCAGTCAATGAA
  AACCAACCTCGTACTGTCCAATATCTACATTTGGGGAAACAAATTTGATGAGGATACCTGTGTGGCATAT
  TCAGATTTGATAAAGAGTGGCCGACTAAAGCCAGATAATACAGATGTGGAACCGTATATGGTGGACGAAC
  ATATTTACCTTTCAGAAGTCTCCAACGGCCTTAAAAGGCATTATTACTGGGCACCGACTTATGGAGAAAC
  TTACATGCCGTCATCTTCCGCAGGCTTTGCTCTTGTTCCTGTGGGCGAACATCTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027941
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027941.1, NP_082217.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1248 bp
• Locus ID: 71827
• UniProt ID: Q9DAM1
• Cytogenetics: 3 A3
• Gene Summary: Highly expressed in stem cells where it may be involved in regulation of pluripotency. In embryonic stem cells (ESCs), important for normal expression of the pluripotency regulators POU5F1/OCT4 and KLF4. Also important for expression of the ectodermal marker gene NES and the endodermal marker gene GATA4. Promotes stem cell proliferation in vitro.[UniProtKB/Swiss-Prot Function]