Eef2kmt (NM_027446) Mouse Untagged Clone

CAT#: MC211076

Fam86 (untagged) - Mouse family with sequence similarity 86 (Fam86), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef2kmt
• Synonyms: 5730409G15Rik; eEF2-KMT; Fam86; Fam86a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211076 representing NM_027446
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTGAGGACCACGAGGGGGCCACGTCTCTGCTGCAGAGTTTCGAGCGCCGCTTCCTGGCGGCTC
  GCGCGCTGCCCTCCTTTCCCTGGCAGAGCCTAGAAGAAAAACTAAAGGACCCCTCGGGTTCTGAGCTGTT
  GCTGGCTATTCTGCAGAGGACTGTGAAGCACCCTGTGTGTGTGCAGCACGGACCTTCTGTGAAGTATGCC
  CGCTGCTTTCTCTCAAAGCTCATCAAAAAGCATGAGGCTGTACCCACGGAGCCTTTGGATGCACTGTATG
  AGGCGTTGGCAGAGGTTCTGATGACCCAAGAGTCCACCCAGTGCCACCGGAGCTATTTGCTGCCTTCAGG
  AAACTCAGTTACACTCTCTGAGAGCACTGCCATCGTGTCCCATGGCACCACAGGCTTGGTTACATGGGAC
  GCTGCTCTCTACCTGGCAGAATGGGCCATTGAGAACCCAGCAGCCTTCACTGACAGGACTATCCTGGAGC
  TAGGCAGTGGAGCTGGCCTTACAGGCCTGGCAATTTGCAAGGCATGCTGCCCCAGAGCATACATCTTCAG
  CGACTGTCATGCCCAAGTGCTTGAGCAGCTCCGTGGAAACGTTCTCCTCAACGGCTTCTCCTTAGAGCCG
  CACACCCCCATTGACGCAGGCAGCTCGAAGGTGACAGTGGCTCAGCTGGACTGGGACGAGGTGACAGCCT
  CTCAGCTCTCTGCCTTCCAGGCAGATGTTGTCATTGCAGCAGATGTACTGTACTGCTGGGAGATGACACT
  GTCACTGGTCAGGGTCCTGAAGATGCTCGAGGACTGCCAGAGGAAGAGCGCTCCTGATGTCTATGTAGCC
  TATACTATCCGCAGCCAGGACACAGGCAAGCTGTTCATCGAAGAGCTGGACCGTGCTGGAATCTACTGGG
  AAGAGGTGCCCCCTCACACTGGCAAACTGTTCCCTTACGAAGAGCATTCAGCCATCGTAATCCTGAAGCT
  GGTGCTGACCAGTCGCCATGGTGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027446
• Insert Size: 1008 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027446.2, NP_081722.1
• RefSeq Size: 2140 bp
• RefSeq ORF: 1008 bp
• Locus ID: 70511
• UniProt ID: Q3UZW7
• Cytogenetics: 16 2.51 cM
• Gene Summary: Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.[UniProtKB/Swiss-Prot Function]