Psmg4 (NM_001110515) Mouse Untagged Clone

CAT#: MC210982

Psmg4 (untagged) - Mouse proteasome (prosome, macropain) assembly chaperone 4 (Psmg4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Psmg4
• Synonyms: mPAC4; PAC-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210982 representing NM_001110515
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGTCTCGGGCCGCTGCGGACGCGGACGTGTCGCTTCACAACTTCAGTGCAAGGCTGTGGGAGC
  AGCTGGTCCACTTTCATGTCATGCGGCTGACGGATTCGCTCTTCCTGTGGGTGGGAGCAACGCCGCATCT
  ACGCAACCTCGCAGTGGCCATGTGCAGCCGCTACCCCGGAAGACCAGCAAACAGGTGTTTGTCAGCTACA
  ACCTCTCCAACACAGACAGTAACTTCACGTTACTCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110515
• Insert Size: 249 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110515.2, NP_001103985.1
• RefSeq Size: 501 bp
• RefSeq ORF: 249 bp
• Locus ID: 69666
• Cytogenetics: 13 A3.3
• Gene Summary: Chaperone protein which promotes assembly of the 20S proteasome.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) lacks an exon in the central coding region which results in a frameshift, compared to variant 2. The encoded isoform (a) is shorter and has a distinct C-terminus, compared to isoform b.