Hfe2 (NM_027126) Mouse Untagged Clone

CAT#: MC210967

Hfe2 (untagged) - Mouse hemochromatosis type 2 (juvenile) (human homolog) (Hfe2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hfe2
• Synonyms: 2310035L15Rik; 5230400G09Rik; AI414844; AI789733; DL-M; hemojuvelin; HJV; Rgmc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210967 representing NM_027126
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGTCCCCTAGTCCCCGGTCCCCCCACGGCAGCCCTCCAACTCTAAGCACCCTCACTCTCCTGC
  TGCTCCTCTGTGGACAGGCTCACTCCCAGTGCAAGATCCTCCGCTGCAATGCCGAGTATGTCTCGTCCAC
  TCTGAGTCTTCGGGGAGGTGGCTCACCGGACACGCCGCGTGGAGGCGGCCGTGGTGGGCTGGCCTCAGGT
  GGCTTGTGTCGCGCCCTGCGCTCCTACGCTCTCTGCACGCGGCGCACGGCCCGCACCTGCCGCGGGGACC
  TTGCTTTCCACTCTGCGGTGCATGGCATAGAGGACCTGATGATCCAGCACAACTGCTCACGCCAGGGTCC
  CACGGCCCCGCCCCCGGCCCGGGGCCCCGCCCTGCCCGGGGCCGGGCCAGCGCCCCTGACCCCAGATCCC
  TGTGACTATGAGGCCCGGTTTTCCAGGCTGCACGGTCGAGCCCCGGGCTTCTTGCATTGCGCATCCTTTG
  GAGATCCCCATGTGCGCAGTTTCCACAACCAATTTCACACATGCCGTGTCCAAGGAGCTTGGCCCTTGCT
  AGATAACGACTTCCTCTTTGTCCAGGCCACCAGCTCCCCGGTTTCGTCGGGAGCCAACGCTACCACCATC
  CGGAAGATCACTATCATATTTAAAAACATGCAGGAATGCATTGACCAGAAAGTCTACCAGGCTGAGGTGG
  ACAATCTTCCTGCAGCCTTTGAAGATGGTTCTATCAATGGGGGCGACCGACCTGGGGGCTCGAGTTTGTC
  CATTCAAACTGCTAACCTTGGGAGTCACGTGGAGATTCGAGCTGCCTACATTGGAACAACTATCATCATT
  CGACAGACAGCTGGGCAGCTCTCCTTCTCCATCAGGGTAGCAGAGGATGTGGCGCGGGCCTTCTCCGCAG
  AGCAGGACCTACAGCTGTGTGTTGGGGGATGCCCTCCGAGCCAGCGACTCTCTCGCTCAGAGCGCAACCG
  CCGTGGGGCTATAGCCATAGATACTGCCAGAAGGCTGTGTAAGGAAGGGCTTCCGGTTGAAGATGCCTAC
  TTCCAATCCTGCGTCTTTGATGTTTCAGTCTCCGGTGACCCCAACTTTACTGTGGCAGCTCAGACAGCTC
  TGGACGATGCCCGAATCTTCTTGACGGATTTAGAGAACTTACATCTCTTTCCCTCAGATGCGGGGCCTCC
  CCTCTCTCCTGCCATCTGCCTAGTCCCGCTTCTTTCGGCCCTCTTTGTTCTGTGGCTTTGCTTCAGTAAG
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027126
• Insert Size: 1263 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027126.4, NP_081402.3
• RefSeq Size: 2021 bp
• RefSeq ORF: 1263 bp
• Locus ID: 69585
• UniProt ID: Q7TQ32
• Cytogenetics: 3 F2.1
• Gene Summary: Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.[UniProtKB/Swiss-Prot Function]