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Dtwd2 (NM_001170960) Mouse Untagged Clone

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SKU
MC210803
Dtwd2 (untagged) - Mouse DTW domain containing 2 (Dtwd2), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dtwd2
Synonyms 1190002H09Rik; 8030470C17Rik; AI414673; BB115449
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC210803 representing NM_001170960
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGCAGGCCGAGGAGCGAACTCTCGGGGAGCCTGCGCCTCCGCCTTCTGGCGCCTTGGCGTCAC
CGACGCCGGACGAAGAGGAGCGGACCGAGGGCGGCGCACCGCCGACGGCGACTCCCGCGGGCGCATCGGG
GGACAGCACGAGTGCAGACGGGCTCTGGGGGCTGCCGGTGGAGCACGCGGAGCGCAGGCCCGAGTGCGGC
CGCTGCAGCCGGCCCCAGAAAGTGTGCTTATGTCCATATCTCCCAGTGCGCCCACTCCAGATCTCTACCC
ACTTGTACATAATCCAGCATCCAGCAGAGGTGCAGTTAAAAACTAGCGTTTGTAGTCAGTATGTAATTCG
GATGCAACCAACAAACAGGTGCCTTTCTACCCTGGAGTGCGCTGCTGTTGCTCTTTCCATCTTGGAGAAA
AACAATTGCATACAAGAGACCCTGCTCCGCCCCCTTCAAGCTCTGTGTTCTTTCCAGCTTCAGCATGGCG
CTCAGATTCGCCTCAGCAAGGAATATCTTCTGAGAAACGGATTGTATCCCAAGCCAATGCCAAAGAACAA
ACGCAAACTCAGGAAGATGGAGCTGTTGATGAACAGTGTTAAAATTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001170960
Insert Size 609 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001170960.1, NP_001164431.1
RefSeq Size 2857 bp
RefSeq ORF 609 bp
Locus ID 68857
UniProt ID Q9D0U1
Cytogenetics 18 D1
Summary Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20a in the D-loop of several cytoplasmic tRNAs (acp3U(20a)). Also has a weak activity to form acp3U at position 20 in the D-loop of tRNAs (acp3U(20)).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks two in-frame exons in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
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"NM_001170960" in other vectors (4)
SKU Description Size Price
MG216268 Dtwd2 (tGFP-tagged) - Mouse DTW domain containing 2 (Dtwd2) transcript variant 2, (10ug) 10 ug
$500.00
MR216268 Dtwd2 (Myc-DDK-tagged) - Mouse DTW domain containing 2 (Dtwd2), transcript variant 2 10 ug
$289.00 $300.00
MR216268L3 Lenti ORF clone of Dtwd2 (Myc-DDK-tagged) - Mouse DTW domain containing 2 (Dtwd2), transcript variant 2 10 ug
$600.00
MR216268L4 Lenti ORF clone of Dtwd2 (mGFP-tagged) - Mouse DTW domain containing 2 (Dtwd2), transcript variant 2 10 ug
$600.00

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