Rnf166 (NM_001033142) Mouse Untagged Clone

CAT#: MC210783

Rnf166 (untagged) - Mouse ring finger protein 166 (Rnf166), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf166
• Synonyms: 1110031E24Rik; AW555115; Zfp313l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210783 representing NM_001033142
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGTTCCGCAGCCTGGTGGCCTCGGCGCAGCAGCGGCAGCCGCCGGCTGGGCCCGCGGGCGGCG
  ACAGCGGCCTGGAGGCGCAGTTCAGCTGTCCCATCTGCCTGGAGGTGTACCATCGGCCAGTGGCCATCGG
  CAGCTGCGGCCACACGTTCTGCGGGGAGTGCCTCCAGCCATGTCTGCAGGTGCCGTCCCCTCTGTGCCCA
  CTCTGCCGTCTGCCCTTCGACCCCAAGAAAGTGGACAAGGCCACCCATGTAGAGAAGCAACTCTCATCCT
  ACAAGGCACCCTGTCGGGGTTGCAACAAGAAGGTGACACTGGCCAAGATGAGAGCGCACATTTCCTCCTG
  CCTGAAGGTCCAGGAGCAGATGGCTAACTGCCCCAAATTCGTCCCTGTGGTGCCCACATCTCAGCCCATC
  CCCAGCAATATCCCCAACCGGTCTACCTTCGCCTGCCCCTACTGCGGAGCCCGCAATCTGGATCAGCAGG
  AGCTGGTGAAGCACTGCGTGGAAAGTCACCGCAGTGATCCCAACCGTGTGGTATGCCCCATCTGCTCAGC
  AATGCCCTGGGGGGACCCCAGCTACAAAAGCGCCAACTTCCTGCAGCATCTGCTGCACCGGCACAAGTTC
  TCCTACGACACCTTCGTGGACTACAGCATCGACGAAGAAGCTGCCTTCCAGGCTGCCCTGGCTCTGTCCC
  TCTCTGAGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033142
• Insert Size: 714 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033142.2, NP_001028314.1
• RefSeq Size: 1783 bp
• RefSeq ORF: 714 bp
• Locus ID: 68718
• UniProt ID: Q3U9F6
• Cytogenetics: 8 E1
• Gene Summary: E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates. In turn, participates in different biological processes including interferon production or autophagy. Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6. Plays also a role in the early recruitment of autophagy adapters to bacteria. Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication.[UniProtKB/Swiss-Prot Function]