Rtn4 (NM_194053) Mouse Untagged Clone

CAT#: MC210753

Rtn4 (untagged) - Mouse reticulon 4 (Rtn4), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rtn4
• Synonyms: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210753 representing NM_194053
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATAGACCAGTCGTCGCTGGTCTCCTCGTCCGCGGATAGCCCGCCCCGGCCCCCGCCCGCTT
  TCAAGTACCAGTTCGTGACGGAGCCCGAGGACGAGGAGGACGAGGAAGACGAGGAGGAGGAGGAGGACGA
  CGAGGACCTGGAGGAATTGGAGGTGCTGGAGAGGAAGCCCGCAGCCGGGCTGTCCGCGGCTCCGGTGCCC
  CCCGCCGCCGCACCGCTGCTGGACTTCAGCAGCGACTCGGTGCCCCCCGCGCCCCGCGGGCCGCTGCCGG
  CCGCGCCCCCCACCGCCCCTGAGAGGCAGCCGTCCTGGGAACGCAGCCCCGCGGCGTCCGCGCCATCCCT
  GCCGCCCGCTGCCGCAGTCCTGCCCTCCAAGCTCCCGGAGGACGACGAGCCTCCAGCGCGGCCTCCGGCG
  CCAGCCGGCGCGAGCCCCCTAGCGGAGCCCGCCGCGCCCCCTTCCACGCCGGCCGCGCCCAAGCGCAGGG
  GCTCGGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCTGC
  AGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTG
  CTGCTGTCTCTGACAGTGTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGA
  CTATCAGCTTTAGGATATATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAG
  GGCATATTTGGAATCTGAAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGT
  CATGTGAACAGCACAATAAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGT
  TTGCAGTGTTGATGTGGGTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGC
  TCTGATCTCACTCTTCAGTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGA
  CTTGCAAACAAGAGCGTTAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAG
  CAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_194053
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194053.3, NP_918942.1
• RefSeq Size: 3804 bp
• RefSeq ORF: 1128 bp
• Locus ID: 68585
• UniProt ID: Q99P72
• Cytogenetics: 11 A3.3
• Gene Summary: Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two alternate exons in the coding region, compared to variant 1. The resulting protein (isoform B2) is shorter when it is compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.