Cela3b (NM_026419) Mouse Untagged Clone

CAT#: MC210641

Cela3b (untagged) - Mouse chymotrypsin-like elastase family, member 3B (Cela3b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cela3b
• Synonyms: 0910001F22Rik; 2310074F01Rik; AI504000; Ela3; Ela3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210641 representing NM_026419
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGACTGCTGAGTTCCCTCCTGCTTGTGGCCCTTGCCTCAGGCTGTGGACAGCCTTCCCACAACC
  CCTCCAGCCGAGTTGTCAATGGCGAGGAAGCTGTTCCCCACAGCTGGCCCTGGCAGGTCTCCCTTCAGTA
  TGAGAAGGATGGGAGCTTCCACCATACCTGTGGTGGCTCCCTCATTACCCCTGACTGGGTTCTGACTGCG
  GGCCACTGCATCTCGACTTCTCGCACCTACCAGGTTGTACTTGGGGAGCATGAACGTGGTGTGGAGGAAG
  GCCAAGAACAGGTGATCCCCATCAACGCTGGAGACCTCTTTGTGCACCCCAAGTGGAACTCTATGTGTGT
  GAGCTGTGGCAATGACATCGCCCTGGTCAAGCTCTCAAGAAGTGCCCAGCTGGGAGATGCAGTCCAGCTT
  GCCTGCCTCCCTCCTGCTGGTGAAATCCTACCCAACGGAGCACCCTGTTACATCAGCGGCTGGGGCCGGC
  TCTCCACCAATGGACCTCTGCCAGACAAGCTGCAGCAGGCCTTGCTGCCTGTGGTGGACTATGAACACTG
  CTCCAGGTGGAACTGGTGGGGCTTGTCTGTGAAGACGACCATGGTGTGCGCCGGCGGCGATATCCAGTCT
  GGCTGCAACGGTGACTCTGGAGGACCCCTCAACTGTCCCGCTGACAATGGCACCTGGCAGGTCCACGGTG
  TGACCAGCTTTGTGTCCTCCTTGGGCTGCAACACCCTGAGGAAGCCCACAGTGTTCACCCGTGTCTCAGC
  CTTCATTGACTGGATTGAGGAGACCATTGCCAACAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026419
• Insert Size: 810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026419.2, NP_080695.1
• RefSeq Size: 897 bp
• RefSeq ORF: 810 bp
• Locus ID: 67868
• UniProt ID: Q9CQ52
• Cytogenetics: 4 69.9 cM
• Gene Summary: Efficient protease with alanine specificity but only little elastolytic activity.[UniProtKB/Swiss-Prot Function]