Prpf18 (NM_026045) Mouse Untagged Clone

CAT#: MC210508

Prpf18 (untagged) - Mouse PRP18 pre-mRNA processing factor 18 homolog (yeast) (Prpf18), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prpf18
• Synonyms: 2810441A10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210508 representing NM_026045
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATCCTGAAATCCGAGATTCTGCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGCTGGTGGAAA
  ATAAAAAATACTTCAAGCGGAGTGAACTGGCGAGAAAAGAAGAGGAAGCGTATTACGAAAGATGTGGCTA
  CAAGATACAGCCAAAAGAGGATGACCAGAAACCACTAACATCGTCAAATCCAGTGCTGGAACTTGAACTA
  GCAGAGGAAAAACTTCCTATGACTCTTTCTAGGCAAGAGGTCATCAGAAGACTGAGAGAAAGAGGAGAGC
  CGATTAGACTGTTTGGGGAAACCGACTATGATGCTTTCCAGCGTTTAAGGAAGATAGAGATTCTTACACC
  AGAAGTTAACAAGGGCTTAAGGAATGATCTGAAAGCAGCTTTGGACAAGATTGACCAGCAGTACTTGAAT
  GAGATTGTGGGTGGTCAGGAACCTGGAGAGGAGGACACCCAGAATGACCTGAAAGTTCACGAGGAAAACA
  CCACCATTGAAGAATTAGAGGCACTGGGGGAGTCCTTAGGGAAAGGTGACGATCATAAGGACATGGACAT
  CATCACCAAGTTCCTGAAGTTTCTTCTTGGTGTTTGGGCTAAAGAACTGAATGCCAGAGAGGACTACGTG
  AAACGCAGTGTGCAGGGCAAACTGAACAGTGCTACCCAGAAGCAGACGGAGTCCTACCTCAGACCGCTTT
  TCAGAAAGCTCCGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACAGATATTATTAAGTTCAT
  GTTGCAGAGAGAGTACGTGAAGGCGAATGATGCTTACCTTCAGATGGCCATTGGCAATGCCCCCTGGCCT
  ATCGGTGTCACCATGGTTGGTATCCATGCCAGAACTGGCAGGGAGAAGATCTTTTCCAAGCATGTTGCGC
  ATGTTTTAAATGATGAAACCCAACGGAAATACATTCAGGGACTGAAGAGGTTAATGACAATTTGCCAGAA
  GCACTTTCCTACAGATCCGTCAAAATGTGTGGAGTATAATGCACTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026045
• Insert Size: 1029 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026045.3, NP_080321.2
• RefSeq Size: 3431 bp
• RefSeq ORF: 1029 bp
• Locus ID: 67229
• UniProt ID: Q8BM39
• Cytogenetics: 2 A1
• Gene Summary: Participates in the second step of pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.