Plekho1 (NM_023320) Mouse Untagged Clone

CAT#: MC210507

Plekho1 (untagged) - Mouse pleckstrin homology domain containing, family O member 1 (Plekho1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plekho1
• Synonyms: 2810052M02Rik; CKIP-1; Ckip1; JZA-20; Jza2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210507 representing NM_023320
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGAGCGGCTCCGGCAAGCGGGGGCCTCCGGATGGAAACCATCAGTCTGCGGCGCCCGAGAAGG
  TCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGTGCT
  GAAAGGCGACCAGCTCTACGTCTCCGAGAAGGAGGTAAAAGATGAGAAAAACAGTCAGGAGGTGTTTGAC
  CTGAGTGACTATGAGAAGTGCGAAGAGCTCCGGAAATCCAAGAGCAGGAGCAAGAAAAATCACAGCAAGT
  TCACCCTGGCCCGGTGCAGACAGCCGGGCACCACGGCACCCAACCTCATCTTCCTGGCCGTGAGTCCTGA
  AGAGAAGGAGTCATGGATCAACGCCCTGAGTTCTGCCATTACCAGAGCTAAAAACCGTATCTTGGATGAG
  GTCACCGTTGAGGAGGACAGCTATCTTGCCCACCCTACTCGAGACAGAGCAAAAATCCAACACTCCCGCC
  GTCCTCCAACCCGGGGACACCTCATGGCTGTGGCTTCGACCTCTACCTCAGATGGGATGCTAACATTAGA
  CCTGATCCAAGAGGAAGACCCTTCCCCTGAGGAGCCAGCCTCTTGTGCTGAGAGCTTCCGGGTCGATCTG
  GACAAGTCTGTGGCCCAGCTGGCTGGCAGTCGACGGAGAGCGGACTCAGACAGGATCCAGCCCTCTTCGC
  AGCGGGCAAGCAGCCTTTCTCGGCCTTGGGAAAAACCAGACAAGGGAGCCCCCTACACCCCACAAGCACT
  GAAGAAGTTCCCCAGTACAGAGAAGAGCCGATGTGCCTCCCTGGAGGAGATCCTATCCCAGAGGGACACT
  GCCCCAGCCCGCCCCCTTCACCTTCAAGCTGAGGAATCCCTACCCCCTGTCCCTGCCCAGCCAGGGCAGC
  TGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGAAGACTCAGGAGCTGCTGGCAGAGGTTCAGGG
  ACTGGGTGACGGCAAGCGGAAGGCCAAGGACCCCCCACAGTCTCCACCCGACTCGGAGTCGGAGCAGCTG
  CTGCTGGAGACAGAGAGGCTGCTGGGAGAGGCTTCCTCCAACTGGAGCCAGGCAAAGAGAGTGCTGCAGG
  AAGTCCGGGAGCTGAGAGACCTGTACAGGCAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACAGAC
  CTCCCAGCACAGTCAATACCGGAAGAGCCTGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023320
• Insert Size: 1227 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC117003, AAI17004
• RefSeq Size: 1301 bp
• RefSeq ORF: 1227 bp
• Locus ID: 67220
• UniProt ID: Q9JIY0
• Cytogenetics: 3 F2.1
• Gene Summary: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]