Naaa (NM_001163687) Mouse Untagged Clone

CAT#: MC210488

Naaa (untagged) - Mouse N-acylethanolamine acid amidase (Naaa), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Naaa
• Synonyms: 2210023K21Rik; 3830414F09Rik; Asahl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210488 representing NM_001163687
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCCTAGCCACCAGGGCCGCGTGCCATGGGGCACACCTGGCCCTGGCTCTGTTGCTGCTACTGT
  CGCTGTCCGGTCCCTGGCTGTCGGCTGTCGTCCCCGGGACTCCGCCTCTCTTCAACGTCAGTCTGGACGC
  GGCCCCGGAGCAGCGCTGGCTGCCGATGCTGCGGCACTACGACCCAGACTTCTTGCGCACCGCGGTGGCG
  CAGGTCATTGGGGTTCCCCAGTGGGTACTCGGGATGGTCGGAGAAATTGTGTCGAAGGTGGAGAGCTTCC
  TGCCTCAGCCCTTCACCGACGAGATCCGCAGCATCTGTGACTCGCTCAACCTCAGCCTGGCTGACGGTAT
  CTTGGTCAACCTGGCCTACGAGGCTTCCGCATTCTGCACCAGTATTGTGGCCCAAGACTCCCAAGGCCAT
  ATTTACCACGGCCGGAACCTGGACTATCCTTTTGGAAAAATCTTACGAAAGCTGACAGCGAACGTGCAGT
  TTATAAAGAATGGGCAGATTGCATTCACTGGGACCACTTTTGTTGGCTATGTAGGACTGTGGACAGGCCA
  AAGTCCACACAAGTTTACAATTTCTGGTGATGAACGAGATAAAGGCTGGTGGTGGGAGAATATGATCGCG
  GCGCTTTCTCTGGGACACTCCCCCATCAGCTGGCTTATCCGCAAAACCCTGAGTGAGTCAGAAAGCTTCG
  AAGCAGCTGTCTACACGCTGGCCAAGACTCCCCTCATTGCTGACGTTTATTACATTGTTGGGGGTACATC
  GCCCAAGGAGGGAGTAGTCATCACCAGGGACCGAGGTGGCCCGGCAGACATTTGGCCTCTTGACCCTCTG
  AATGGAGAGTGGTTCCGAGTTGAGACAAATTATGACCATTGGAAGCCTGCACCCAAGGTGGATGACCGAA
  GAACACCAGCCATCAAAGCCCTAAATGCTACAGGGCAAGCTCACCTCAATCTGGAGACCCTCTTCCAGGT
  TTTATCCCTGTTTCCTGTTTATAACAACTACACAATTTATACTACAGTGATGAGCGCTGCCGAGCCCGAC
  AAGTACCTGACCATGATCAGAAACCCGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163687
• Insert Size: 1083 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163687.1, NP_001157159.1
• RefSeq Size: 2418 bp
• RefSeq ORF: 1083 bp
• Locus ID: 67111
• UniProt ID: Q9D7V9
• Cytogenetics: 5 E2
• Gene Summary: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-stearoylethanolamine > N-oleoylethanolamine > N-linoleoylethanolamine > N-arachidonoylethanolamine.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1.