Upf3a (NM_025924) Mouse Untagged Clone

CAT#: MC210473

Upf3a (untagged) - Mouse UPF3 regulator of nonsense transcripts homolog A (yeast) (Upf3a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Upf3a
• Synonyms: 2600001C03Rik; 4930546M19Rik; RENT3A; UPF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210473 representing NM_025924
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCGGAAGAGGGGGCCGGAGGCTTAGGCGCAGCCCTGGCTGCGCGGGGCCCGAGCTGGAGGGAGA
  AACTATCGAGCTCAGAGACCCACTGCCGCCGCGAGTCACCGCGGAAGGAGTCGGCGGCGCCTCCGGCCCC
  GACCCTAAGTGAGAGCGGGGCCGGCAAGCCTCGCGAGGAGAAGAGGACAGCCCTGAGCAAGGTGGTCCTC
  CGGCGTCTGCCCCCGGGCCTCACCAAAGAGCAGCTGGAAGAGCAACTGCGCCCCCTGCCGGCGCACGATT
  ACTTCGAGGTGGTGGCTGCGGACCTCAGCCTTTATCCTCATGTCTACTCCAGAGCATACATTAATTTTAG
  GAACCCTGATGACATCCTGCTGTTCAGAGACCGTTTTGACGGCTATATCTTCATTGGCAATAAAGGCCTG
  GAGTATCCTGCTGTGGTGGAGTTTGCTCCATTCCAGAAGATTGCCAAAAAGAAGCTGAAGAAAAAAGATG
  CCAAGACCGGAAGCATTGAAGATGACCCAGAATATAAACAGTTCCTGGAGTCGTACAGCCTGGAGGAGGA
  GAAGACCAGCGCTAGTCCCGAGACGCTGCTGGGAGAGATAGAAGCCAAGACGAGAGAGCTCCTGGCTAGA
  AGGACCACCCCTCTTTTGGAATATATTAAAAACAGAAAATTAGAAAAGCAGAGACTCCGGGAAGAAAAGC
  GCGAGGAGCGGAGGAGGAGGGAGCTAGAAAAGAAGCGTCTGCGGGAAGAAGAGAAGAGGAAGAGGAGAGA
  GGAGGACCGCTGTAAGAGGAAGGAGGCGGAGAGGCACAAGAGAGCGGAGAAGGACGTGAGGATCAAGCTT
  CTTAAAAAACTGGAAACGGGAGAGGAAGTGGCCACAGAGAAACCCAAAGAAAGAGGTGAAGCTGTTGGTG
  CTGGAGACGAGAAGCAGGAAGACCGGCTGGTAGCGAGCCAGCCAGAGACAGCTGGGGAGAGGCTACAGGA
  TACCAGTGATAAAGAGCCAAGGGACAAGAGATCTCGAGAGAAAGACCCTGAAACTTCTAGATGTCATCCG
  GACACCTGCAGGAAGCACAGCAGTCACTCAGAGTTTGCCAGGTTCTCCAGAAGGAATGAAGACGAGGCAA
  AGTGGGGGAAAGGCTTCGCCCAGGACAGAGGGAAGAAGGGCCAGGATGGCAGTGTCCCCATGGACGTGGC
  AGAGAGAAGCGGCAAGGAGCACAAGGAGTATGACAGCCTGGGGCCAAGAAAGGATCGCCTGGGACACAAG
  GCCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025924
• Insert Size: 1269 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025924.2, NP_080200.1
• RefSeq Size: 1402 bp
• RefSeq ORF: 1269 bp
• Locus ID: 67031
• Cytogenetics: 8 A1.1