Acad8 (NM_025862) Mouse Untagged Clone

CAT#: MC210458

Acad8 (untagged) - Mouse acyl-Coenzyme A dehydrogenase family, member 8 (Acad8), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acad8
• Synonyms: 2310016C19Rik; AI786953
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210458 representing NM_025862
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGTTGCGGTCCGGATACAGGCGCTTTGGCTGTCTGCGCGCGGCACTCAAGTCCCTGGCTCAGA
  CCCACCACCGGAGCATCACCTTCTGCATCGATCCTTCCTTGGGGCTAAATGAAGAACAGAAAGGATTTCA
  GAAAGTGGCCTTTGACTTTGCAGCTCGGGAAATGGCTCCCAATATGGCGGAGTGGGATCAGAAGGAGCTG
  TTCCCTGTGGATGTGATGCGGAAGGCTGCACAGCTGGGATTTGGGGGGGTCTATGTGCGAACAGATGTGG
  GTGGATCTGGACTGTCTCGCCTTGATACTTCTGTCATCTTTGAAGCCTTGGCCACAGGCTGCACCAGCAC
  CACTGCCTATATAAGCATCCACAACATGTGTGCCTGGATGATTGATAGCTTTGGAAATGAGGAACAGAGG
  CACAAATTTTGCCCACCGCTCTGTACCATGGAGAAGTTTGCTTCCTACTGCCTCACTGAACCAGGCAGTG
  GGAGTGATGCTGCATCTCTTCTGACCTCAGCTAAACAACAAGGAGATCATTACATCCTCAATGGTTCCAA
  GGCCTTCATCAGTGGGGGAGGTGAGTCAGACATCTATGTGGTCATGTGCCGAACTGGTGAGTCTGGCGCC
  AAAGGTATCTCCTGCATAGTTGTTGAGAAAGGAACCCCTGGTCTCAGCTTTGGCAAGAAGGAGAAGAAGG
  TGGGGTGGAACTCGCAGCCAACCCGAGCAGTGATCTTTGAAGACTGTGCTGTCCCTGTGGCCAACAGGAT
  TGGGACCGAGGGGCAGGGTTTTCTCATCGCCATGAAAGGACTGAATGGTGGAAGGATCAATGTTGCATCC
  TGCTCTCTGGGAGCCGCTCATGCTTCAGTTATCCTAACCCAAGAACACCTCAAGGTCCGCAAGCAGTTTG
  GAGCACCTTTGGCCAGAAGCCAGTACCTCCAATTCCAGTTGGCAGATATGGCCACCAAGCTAGTTGCTTC
  CCGGCTGATGATCCGTACTGCAGCAGTGGCTTTGCAGGAAGAGCGGGAAGATGCAGTGGCCCTGTGTTCC
  ATGGCCAAGCTCTTTGCTACAGAGGAGTGCTTTGCTATCTGCAACCAGGCTTTGCAGATGCATGGGGGCT
  ACGGCTATCTGAAGGACTATGCTGTTCAACAGTATATGCGGGACTCCAGGGTCCACCAGATCCTAGAAGG
  TAGCAATGAAGTGATGAGGATGCTGATCTCTCGAAACCTGCTTCAGGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025862
• Insert Size: 1242 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025862.2, NP_080138.2
• RefSeq Size: 2865 bp
• RefSeq ORF: 1242 bp
• Locus ID: 66948
• UniProt ID: Q9D7B6
• Cytogenetics: 9 A4
• Gene Summary: Has very high activity toward isobutyryl-CoA. Is an isobutyryl-CoA dehydrogenase that functions in valine catabolism (By similarity).[UniProtKB/Swiss-Prot Function]