Aspn (NM_001172481) Mouse Untagged Clone

CAT#: MC210418

Aspn (untagged) - Mouse asporin (Aspn), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aspn
• Synonyms: 4631401G09Rik; AA986886; PL; Plap1; SL; Slrr1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210418 representing NM_001172481
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGTATGTGATGCTACTGCTTTTGGCTGTGTGCTCTGCCAAACCCTTCTTTAGCCCTTCCCACA
  CAGCACTGAAGAATATGATGTTGAAGGATATGGAAGACACAGATGATGACGATAACGATGATGACGACAA
  CTCTCTTTTTCCAACGAAAGAGCCAGTGAACCCTTTTTTCCCTTTCGATTTGTTTCCAACATGTCCATTT
  GGGTGCCAATGTTACTCTCGAGTTGTTCACTGCTCTGATCTAGGTCTGACATCGGTTCCAAACAACATTC
  CATTTGATACTCGAATGGTTGACCTTCAAAATAATAAAATCAAGGAAATTAAAGAAAATGACTTTAAAGG
  ACTCACTTCACTTTATGCTCTGATTCTGAACAACAACAAGCTAACAAAGATTCACCCAAAAACCTTTCTA
  ACCACAAAGAAATTGAGAAGGCTATATTTATCCCACAACCAACTAAGTGAAATTCCACTTAATCTTCCCA
  AATCATTAGCAGAACTCAGAATTCATGATAATAAAGTTAAGAAGATACAAAAGGACACGTTCAAGGGAAT
  GAATGCTTTACATGTTTTGGAAATGAGTGCAAACCCTCTTGAGAACAACGGGATAGAACCAGGGGCATTT
  GAAGGGGTGACAGTATTCCATATCAGGATCGCTGAAGCAAAACTAACCTCAATTCCAAAAGGCCTACCAC
  CAACTTTGCTGGAGCTTCATTTAGATTTTAATAAAATTTCAACGGTGGAACTTGAAGATCTTAAACGGTA
  CAGGGAACTGCAAAGGCTGGGTCTTGGAAACAACAGAATCACAGATATTGAAAATGGAACTTTTGCTAAT
  ATACCACGTGTGAGAGAGATACACTTGGAACACAATAAACTAAAAAAAATCCCTTCAGGATTACAGGAGT
  TGAAATACCTCCAGATAATCTTCCTTCATTATAATTCAATTGCAAAAGTGGGAGTGAATGACTTCTGTCC
  AACAGTGCCAAAGATGAAGAAATCTTTATACAGTGCAATAAGTTTATTCAACAACCCAATGAAGTACTGG
  GAAATACAACCTGCAACATTTCGTTGTGTTCTTGGCAGAATGAGTGTTCAGCTTGGGAATGTTGGAAAAT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172481
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172481.1, NP_001165952.1
• RefSeq Size: 2345 bp
• RefSeq ORF: 1122 bp
• Locus ID: 66695
• UniProt ID: Q99MQ4
• Cytogenetics: 13 A5
• Gene Summary: This gene encodes a member of the small leucine-rich proteoglycan family. The encoded protein is an extracellular matrix protein that modulates the transforming growth factor-beta signaling pathway, regulating cartilage matrix gene expression and cartilage formation. The protein plays a role in the pathology of osteoarthritis. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.