Gemin2 (NM_025656) Mouse Untagged Clone

CAT#: MC210401

Gemin2 (untagged) - Mouse survival of motor neuron protein interacting protein 1 (Sip1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gemin2
• Synonyms: gemin-2; S; Sip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210401 representing NM_025656
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGGGTTCCAGCCGAGTCTGCGGTGGAAGAGTTGATGCCACGGTTGTTACCTGTGGAGCCCTGTG
  ACTTGACGGAAGGTTTCGATCCCTCGGTCCCCCCCAGGACGCCTCAGGAATATCTGAGGAGAGTGCAGAT
  CGAAGCAGCCCAATGTCCAGATGTTGTGGTAGCCCAGATTGACCCAAAGAAGTTGAAAAGGAAGCAAAGT
  GTGAACATTTCTCTTTCCGGATGCCAGCCTGCGCCTGAAGGTTACTCTCCAACACTTCAGTGGCAACAAC
  AACAAGTGGCACATTTTTCAACTGTTCGACAGAGTGTACACAAGCATAGAAATCACTGGAAATCACAACA
  GTTGGACAGTAATGTGGCAATGCCAAAATCTGAAGATGAAGAAGGCTGGAAAAAATTTTGTCTGGGTGAA
  AGGTTATGTGCTGAAGGGGCCACTGGACCGTCTACAGAGGAAAGCCCTGGGATCGATTATGTACAAGTTG
  GTTTTCCTCCTTTGCTTAGTATTGTAAGCAGAATGAATCAGACAACCATTACTAGTGTCTTGGAATATCT
  AAGTAATTGGTTTGGAGAAAGAGACTTTACTCCAGAATTGGGAAGATGGTTTTATGCTTTGTTGGCTTGT
  CTTGAAAAGCCTTTATTACCTGAGGCTCATTCACTAATCCGACAGCTTGCAAGAAGATGTTCTGAAGTGA
  GGCTGCTGGTGGGCAGTAAAGATGACGAAAGAGTCCCAGCTTTGAATCTGCTGATCTGCTTAGTTAGCAG
  GTATTTTGATCAACGCGATTTAGCTGATGAGCCATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025656
• Insert Size: 810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025656.5, NP_079932.2
• RefSeq Size: 1590 bp
• RefSeq ORF: 810 bp
• Locus ID: 66603
• UniProt ID: Q9CQQ4
• Cytogenetics: 12 C1
• Gene Summary: This gene encodes one of the proteins found in the survival of motor neuron (SMN) complex, which consists of the SMN protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal small nuclear ribonucleoproteins (snRNP) and for pre-mRNA splicing. This protein interacts directly with the SMN protein and it is required for formation of the SMN complex. Disruption of this gene in mouse resulted in impaired snRNP assembly, and motor neuron degeneration. [provided by RefSeq, Sep 2015]