Trim13 (NM_023233) Mouse Untagged Clone

CAT#: MC210398

Trim13 (untagged) - Mouse tripartite motif-containing 13 (Trim13), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim13
• Synonyms: 3110001L12Rik; CAR; LEU5; Rfp2; RNF77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210398 representing NM_023233
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTTGAAGAAGACCTCACATGCCCAATTTGCTGCAGTTTGTTTGATGACCCCCGAGTGTTGC
  CCTGCTCACACAACTTCTGCAAAAAATGCTTAGAAGGGCTCTTAGAGGGGAATGTGCGGAATTCCCTGTG
  GAGACCATCTCCCTTCAAGTGTCCTACCTGCCGTAAGGAAACCTCAGCTACTGGAGTCAACAGTCTGCAG
  GTCAATTACTCCCTAAAGGGTATCGTGGAGAAATACAACAAAATCAAGATTTCTCCCAAGATGCCAGTGT
  GCAAAGGACATTTGGGGCAGCCTCTCAACATCTTCTGCGTAACTGATATGCAGCTGATTTGTGGGATCTG
  TGCTACTCGAGGCGAGCACACCAAGCATGTCTTCTCTTCTATTGAAGATGCCTACGCTCGAGAAAAGAAT
  GCCTTTGAGTCCCTCTTTCAGAGTTTCGAGACTTGGCGCCGGGGAGATGCTCTTTCCCGCTTGGATACTT
  TGGAAACAAACAAGAGGAAAGCCCTCCAGTTACTCACGAAGGATTCAGATAAAGTAAAGGAGTTTTTTGA
  GAAGTTACAGCACACCTTGGATCAAAAGAAGAATGAAATCCTGTCTGACTTTGAAACTATGAAGCTTGCA
  GTTATGCAAACCTATGACCCGGAGATCAACAAAATCAACACTATTTTACAGGAGCAGCGGATGGCCTTCA
  ACATTGCTGAGGCTTTCAAAGATGTCTCAGAACCTATTATATTTTTGCAACAGATGCAAGAGTTCAGGGA
  GAAAATCAAAGTAATCAAGGAAACTCCTTTGCCACACTCTAATTTGCCCACAAGCCCTTTAATGAAGAAC
  TTTGATACCAGTCAGTGGGGAGACATTAAACTAGTTGATGTGGATAAACTGTCTTTGCCGCAAGACACAG
  GTGTGTTCACTAGCAAGATTCCCTGGTACCCCTATCTGCTGCTCATGATGGTAGTTCTGCTGGGTCTCCT
  CATATTCTTTGGCCCCACTGTATTCCTGGAATGGTCTCCACTTGATGAATTGGCAACTTGGAAAGACTAT
  CTTTCAAGCTTCAATTCTTACCTGACTAAGTCTGCTGATTTTATAGAACAATCTGTTTTTTACTGGGAAC
  AGATGACAGATGGGTTTTTCATTTTTGGTGAAAGAGTAAAAAATGTTAGTTTGGTGGCACTGAACAATGT
  GGCAGAGTTTATATGCAAATACAAACTATTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023233
• Insert Size: 1224 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023233.3, NP_075722.1
• RefSeq Size: 1578 bp
• RefSeq ORF: 1224 bp
• Locus ID: 66597
• UniProt ID: Q9CYB0
• Cytogenetics: 14 D1
• Gene Summary: Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation. Participates as well in T-cell receptor-mediated NF-kappa-B activation. In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.