Asph (NM_001177852) Mouse Untagged Clone

CAT#: MC210251

Asph (untagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asph
• Synonyms: 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210251 representing NM_001177852
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGATAAAGAGGCAAAGCACGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGT
  CCTTTTTCACATGGTTCATGGTCATTGCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGA
  CTTGGTCGATTATGAAGAAGTTCTAGGAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGAT
  GTGGATGATGCCAAAGTTTTATTAGGCCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGG
  AGGAGGCAGAGACTCACGCTGAGCTGGAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGA
  TGAAGTAAAGGAACAAATTCAGTCCCTTCTTCAGGAATCAGTACACACAGACCATGACCTTGAAGCAGAT
  GGGCTGGCAGGAGAACCACAGCCGGAGGTTGAGGACTTCCTCACAGTGACCGACAGTGATGACAGATTTG
  AGGACCTGGAACCCGGGACAGTTCATGAAGAAATCGAGGATACTTACCATGTGGAAGACACAGCATCGCA
  GAACCATCCAAATGACATGGAAGAGATGACGAATGAGCAGGAAAATTCAGATCCCAGTGAAGCAGTGACA
  GATGCAGGTGTGCTGCTGCCCCATGCAGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAAC
  CCTCAGAGCATGAAGGGGTCGCGATTTCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAAT
  AAATGTCGCCTCTGTTGAAGAACAGCAAGACACACCACCAGATACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177852
• Insert Size: 819 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177852.1, NP_001171323.1
• RefSeq Size: 2726 bp
• RefSeq ORF: 819 bp
• Locus ID: 65973
• UniProt ID: Q8BSY0
• Cytogenetics: 4 A1
• Gene Summary: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) has multiple differences which result in distinct UTRs and coding end regions, compared to variant 1. The resulting protein (isoform 6) is shorter and has distinct N- and C-termini when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.