Tbata (NM_001017419) Mouse Untagged Clone

CAT#: MC210250

Tbata (untagged) - Mouse thymus, brain and testes associated (Tbata), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbata
• Synonyms: 1700021K02Rik; AI428928; S; Spatial; Titest
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210250 representing NM_001017419
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAGAGGTGAACCAGCTGTCTGAGCATCCTCTAGTGAGTCCAAAGGCAGAGCCCCAGCCAGAGA
  CGAAGCCGGAGAACCTTCCAAGGAGCCACGGGGATGTTGGGCTCCAGAAAGAGACTGTGGTCCCAGGCAT
  TGTGGATTTCGAGCTGATCCATGAGGAGCTGAAGACCACAAAGCCCCAAACATCACAACCAACACCCAGT
  GCCTACCGCTTTGGACGCCTAAGCCACCATTCCTTCTTCTCGAGGCACCACCCCCAACCACAGCGAGTGA
  CTCATATCCAAGATATCGCTGGGAAGCCTGTCTGCGTGGTCAGGGACGAGTTCTCTCTGTCGGCCTTGAC
  TCAGCCCACATTCTTATCCCGCTGTCTGATGGGGATGCCCACCATCTCTGTCCCCATTGGGGATCCACAG
  TCCAATCGGAACCCCCAGCTTTCTACTTCTGACACCTGGAGGAAGAAACTGAAGGACCTGGCTTCCCGAG
  TGACTGTCTTCACTAAGGAAATCCAGCCAAAGCCCGATGAGCAGAAGGAAGAGCCACCTCTGAGGGAACC
  TCCTCCGAGAGAGCAGGGGGCCAAATACTCAGCTGAGACCGGTAGGCTTATCCCTGCTTCCAGCCAAGCC
  CTCACCCGTCGCAACCGCCAGGGCCAGCGGGTCCACCCTTCTAGCAAAGATGGAGGAGTCCAAGCCTCCA
  TTCTGCAGGACCAGGAGCTGCTGATTTTGGAGCTCCTTTGTCAAATTCTACAAACAGATTCTCTAAGGGC
  TATTCAGTTCTGGCTGCTTTATGCTCCATCAAAAGAAAAAGATTTAGCTCTGGGGCTTCTGCAAACTGCC
  GTGGCTCAGCTTATCCCCCAGCCCCTTTCCTCCATCCCAGCAGAGAAGCTCTGGAACCATCTCCAAGAGC
  TTCAAGAGCCTCAAGAGACACAAGAGGCAGCCTACAGTCCATCCCTGAAGAAGACTAGGTCACCACCTCT
  ACCCAAAACAGACAAACCAGAGTACATAGGCAAAGCCCAAGTCCTCCTGGTGCATCCCAGCGAGGACCCA
  GAGGAGAAAACGACCAAGGCTGAAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017419
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017419.2, NP_001017419.1
• RefSeq Size: 1443 bp
• RefSeq ORF: 1080 bp
• Locus ID: 65971
• UniProt ID: Q7TSD4
• Cytogenetics: 10 B4
• Gene Summary: This gene encodes a putative transcription factor that is highly expressed in thymic cortical stromal cells, and may be involved in T-cell development. Its expression is developmentally regulated in the testis, where it is restricted to the haploid round spermatids during spermatogenesis, and thus this gene may also have a role in the control of male germ cell development. Alternative splicing of this gene results in two sets of transcript variants: the variants containing 5 additional exons at the 3' end encode long isoforms that are highly expressed in the testis, while the variants lacking the 3' end exons encode short isoforms that are highly expressed in the thymus. Most of the transcripts encoding the short isoforms have been shown to initiate translation from non-AUG (CUG) start sites. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate, in-frame acceptor splice site in one of the coding exons compared to transcript variant 1, resulting in an isoform (2, also known as Spatial-delta) that is missing an internal segment compared to long isoform 1, and like the latter, it is highly expressed in the testis.