Wtap (NM_001113533) Mouse Untagged Clone

CAT#: MC210212

Wtap (untagged) - Mouse Wilms' tumour 1-associating protein (Wtap), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wtap
• Synonyms: 2810408K05Rik; 9430038B09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210212 representing NM_001113533
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAACGAAGAACCTCTTCCTAAAAAGGTCCGACTGAGTGAAACAGACTTCAAAGTTATGGCACGGG
  ATGAGTTAATTCTAAGATGGAAACAATATGAAGCATATGTTCAAGCTTTGGAGGGAAAGTACACAGATCT
  TAATTCAAACGATGTGACTGGTTTAAGGGAATCTGAAGAAAAACTAAAGCAGCAACAGCAGGAGTCTGCA
  CGCAGGGAGAACATTCTTGTCATGCGGCTAGCAACCAAAGAGCAGGAGATGCAAGAGTGCACCACTCAAA
  TCCAGTACCTCAAGCAAGTTCAGCAGCCGAGTGTGGCCCAACTGAGATCAACAATGGTAGACCCAGCAAT
  CAACTTGTTTTTCCTAAAAATGAAAGGTGAACTGGAACAGACTAAAGACAAACTGGAACAAGCCCAAAAT
  GAACTGAGTGCCTGGAAGTTTACGCCTGATAGCCAAACAGGCAAAAAGCTAATGGCGAAGTGTCGAATGC
  TTATCCAGGAGAACCAAGAGCTTGGAAGGCAGCTGTCCCAGGGCCGTATTGCACAGCTTGAAGCAGAATT
  GGCTTTACAGAAGAAATATAGTGAGGAGCTTAAAAGCAGTCAGGATGAACTGAATGACTTCATCATTCAA
  CTTGATGAAGAAGTAGAGGGTATGCAGAGCACCATTCTAGTTCTTCAGCAACAATTGAAGGAGACACGAC
  AGCAGTTGGCACAGTACCAGCAGCAGCAGTCTCAAGCTTCAGCTCCAAGTACCAGCAGGACTACATCTTC
  TGAACCTGTAGATCAGGCAGAGGTCACAAGCAAAGACTGCAGTCGTCTGGCAAATGGACCAAGTAATGGC
  AGCTCCTCCCGCCAGAGGACGTCTGGGTCTGGATTTCACAGGGAGGGGAGCACACCTGAGGATGACTTTC
  CTTCTTCTTCAGGGAATGGTAATAAGGCCTCCAACAGCTCAGAGGAGAGAACTGGCAGAGGAGGTAGTAG
  TTACATAAACCCACTCAGTGCGGGGTATGAAAGTGTAGACTCTCCCACGGGCAGTGAAAACTCTCTCACA
  CATCACTCAAATGACACAGACTCCAGTCATGACCCTCAAGAGGAGAAAGCAGTGAGTGGGAAAGGTAATC
  GAACTGTGGGTTCCCGCCATGTTCAGAATGGCTTGGACTCAAATGTAAATGTACAGGGTGCAGTTTTGTA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113533
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113533.1, NP_001107005.1
• RefSeq Size: 2051 bp
• RefSeq ORF: 1191 bp
• Locus ID: 60532
• Cytogenetics: 17 A1
• Gene Summary: Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3' UTR (PubMed:29547716). Required for accumulation of METTL3 and METTL14 to nuclear speckle (By similarity). Acts as a mRNA splicing regulator (By similarity). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (By similarity). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.