Qtrt1 (NM_021888) Mouse Untagged Clone

CAT#: MC210209

Qtrt1 (untagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Qtrt1
• Synonyms: 2610028E17Rik; Tgt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210209 representing NM_021888
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTAGGCAGCCCAGGTTCGCTTGAGTCCGCTCCACGAATAATGCGGCTGGTCGCTGAGTGCA
  GTCGCTCCGGAGCTCGGGCGGGTGAGCTGCGGCTGCCGCATGGAACGGTAGCCACCCCTGTGTTCATGCC
  TGTGGGTACACAGGCCACCATGAAGGGTATCACCACGGAGCAGCTGGACTCCCTGGGCTGCCGCATCTGC
  TTGGGCAACACCTACCATCTGGGGCTGAGGCCGGGACCGGAGCTGATCCGGAAAGCCCAGGGTCTTCACG
  GCTTCATGAATTGGCCCCACAATCTGCTGACGGATAGCGGCGGCTTCCAGATGGTGTCCCTCTTCTCCCT
  GTCCGAGGTGACGGAGGAGGGCGTCCACTTCCGCTCGCCCTACGATGGAGAAGAGACACTTTTGAGCCCA
  GAGAGGTCGGTGGAGATCCAGAATGCTCTGGGCTCGGACATCATCATGCAGTTAGACCATGTGGTGAGCA
  GCACAGTGACAGGCCCTCTAGTGGAGGAGGCCATGCACAGGTCAGTCCGCTGGCTGGACAGATGCATCGC
  AGCCCATAAGCACCCGGACAAGCAGAACCTCTTTGCCATCATCCAGGGTGGACTGAATGCTGATCTTCGG
  ACCACTTGTCTGAAAGAGATGACCAAGAGAGATGTGCCGGGCTTCGCCATCGGAGGTCTGAGTGGAGGAG
  AAAGCAAGGCACAGTTCTGGAAGATGGTGGCGTTGAGCACCTCCATGCTGCCTAAGGACAAGCCACGATA
  CCTGATGGGGGTTGGCTATGCCACCGATTTGGTGGTCTGCGTGGCTCTTGGATGTGACATGTTCGACTGT
  GTGTACCCCACACGGACAGCGCGCTTTGGATCCGCCCTTGTGCCCACTGGGAACCTTCAGTTGAAGAAGA
  AGCAGTATGCCAAGGATTTCAGCCCCATAAACCCTGAGTGTCCCTGTCCCACCTGCCAGACGCACAGCCG
  AGCCTTCCTGCATGCCCTGCTACACAGTGACAACACTACAGCACTACACCACCTAACCGTGCACAACATT
  GCCTATCAGCTGCAGCTCCTGAGTGCCGTGCGCAGCAGTATCTTGGAACAGCGCTTTCCTGACTTTGTAC
  GAAACTTCATGCGCACTATGTATGGTGACCACAGCCTCTGTCCTGCCTGGGCCGTTGAAGCACTGGCCTC
  TGTGGGAATCATGCTCACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021888
• Insert Size: 1212 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021888.2, NP_068688.2
• RefSeq Size: 1328 bp
• RefSeq ORF: 1212 bp
• Locus ID: 60507
• UniProt ID: Q9JMA2
• Cytogenetics: 9 A3
• Gene Summary: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:19414587, PubMed:29862811). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).[UniProtKB/Swiss-Prot Function]