Tas2r108 (NM_020502) Mouse Untagged Clone

CAT#: MC210121

Tas2r108 (untagged) - Mouse taste receptor, type 2, member 108 (Tas2r108), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tas2r108
• Synonyms: mGR08; mt2r8; T2r8; T2R108; Tas2r4; Tas2r8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210121 representing NM_020502
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGGGAACTGTATGTATTTGTGTTTGCTGCCTCGGTTTTTTTAAATTTTGTAGGAATCATTGCAA
  ATCTATTTATTATAGTGATAATTATTAAGACTTGGGTCAACAGTCGCAGAATTGCCTCTCCGGATAGGAT
  CCTGTTCAGCTTGGCCATCACTAGATTCCTGACTTTGGGGTTGTTTCTACTGAACAGTGTCTACATTGCT
  ACAAATACTGGAAGGTCAGTCTACTTTTCCACATTTTTTCTATTGTGTTGGAAGTTTCTGGATGCAAACA
  GTCTCTGGTTAGTGACCATTCTGAACAGCTTGTATTGTGTGAAGATTACTAATTTTCAACACCCAGTGTT
  TCTCCTGTTGAAACGGACTATCTCTATGAAGACCACCAGCCTGCTGTTGGCCTGTCTTCTGATTTCAGCC
  CTCACCACTCTCCTATATTATATGCTCTCACAGATATCACGTTTTCCTGAACACATAATTGGGAGAAATG
  ACACGTCATTTGACCTCAGTGATGGTATCTTGACGTTAGTAGCCTCTTTGGTCCTGAACTCACTTCTACA
  GTTTATGCTCAATGTGACTTTTGCTTCCTTGTTAATACATTCCTTGAGAAGACATATACAGAAGATGCAG
  AGAAACAGGACCAGCTTTTGGAATCCCCAGACGGAGGCTCACATGGGTGCTATGAGGCTGATGATCTGTT
  TCCTCGTGCTCTACATTCCATATTCAATTGCTACCCTGCTCTATCTTCCTTCCTATATGAGGAAGAATCT
  GAGAGCCCAGGCCATTTGCATGATTATTACTGCTGCTTACCCTCCAGGACATTCTGTCCTCCTCATTATC
  ACACATCATAAACTGAAAGCTAAAGCAAAGAAGATTTTCTGTTTCTACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020502
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020502.1, NP_065248.1
• RefSeq Size: 894 bp
• RefSeq ORF: 894 bp
• Locus ID: 57253
• UniProt ID: Q9JKT3
• Cytogenetics: 6 B1
• Gene Summary: Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency (By similarity).[UniProtKB/Swiss-Prot Function]