Rgs7bp (NM_029879) Mouse Untagged Clone

CAT#: MC209918

Rgs7bp (untagged) - Mouse regulator of G-protein signalling 7 binding protein (Rgs7bp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgs7bp
• Synonyms: A930030I01Rik; AI842293; D13Bwg1146e; R7bp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209918 representing NM_029879
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGCACCGAATGGGCGCAAAAAGCGCCCCAGCCGGTCCACCCGCTCCTCGATCTTCCAGATCA
  GCAAACCTCCGCTGCAGAGCGGGGATTGGGAGCGCAGGGGCAGCGGCTCCGAAAGCGCCCACAAAACCCA
  ACGAGCCCTGGACGACTGCAAGATGCTTGTCCAAGAGTTCAACACGCAAGTGGCCCTGTACCGAGAGTTG
  GTCATTTCTATTGGGGACGTCTCAGTCAGCTGCCCCTCTCTGCGAGCTGAAATGCACAAGACAAGAACCA
  AAGGCTGTGAAATGGCCCGTCAGGCACACCAAAAGCTGGCTGCCATCTCAGGCCCGGAAGATGGTGAGAT
  CCATCCCGAAATCTGCCGGCTTTACATCCAGCTGCAGTGTTGCCTAGAGATGTACACCACAGAGATGCTG
  AAGTCCATTTGTCTGCTGGGGTCTCTTCAGTTTCATCGAAAAGGAAAAGAAGCTAGTGGGGGAGCGAAGA
  ATTTGGACAGCAAAATTGAGGAGAATGCTGAAACACCTGCCCTGGAGGACTCCTTGTCATCCCCTCTCGA
  GAGCCAGCAGCAGTGCTGGCAGGTCGCCACAGATATTGAAAACACTGAAAGAGACATGCGAGAAATGAAA
  AACCTTCTTAGCAAACTCAGGGAAACGATGCCTTTACCACTGAAAAATCAAGATGACAGCAGCCTTCTGA
  ATCTAACGCCCTACCCCATGGTCAGAAGACGGAAGAGAAGGTTCTTCGGCTTATGTTGTCTTGTCTCAAG
  CTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029879
• Insert Size: 774 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029879.2, NP_084155.2
• RefSeq Size: 5828 bp
• RefSeq ORF: 774 bp
• Locus ID: 52882
• UniProt ID: Q8BQP9
• Cytogenetics: 13 56.9 cM
• Gene Summary: Regulator of G protein-coupled receptor (GPCR) signaling. Regulatory subunit of the R7-Gbeta5 complexes that acts by controlling the subcellular location of the R7-Gbeta5 complexes. When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cytoplasmic shuttling. May also act by controlling the proteolytic stability of R7 proteins, probably by protecting them from degradation.[UniProtKB/Swiss-Prot Function]