Scd3 (NM_024450) Mouse Untagged Clone

CAT#: MC209830

Scd3 (untagged) - Mouse stearoyl-coenzyme A desaturase 3 (Scd3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Scd3
• Synonyms: 4930513N16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209830 representing NM_024450
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGGCACCTGCTGCAAGAAGAGATGACGCCTTCGTACACGACCACCACCACCATCACAGCGCCTC
  CCTCTGGAAGCCTGCAGAATGGACGAGAGAAGGTGAAGACGGTGCCCCTCTACCTGGAAGAAGACATCCG
  TCCTGAAATGAAAGAAGATATATACGACCCCACCTATCAGGATGAGGAGGGGCCCCCGCCCAAGCTGGAG
  TACGTCTGGAGGAACATCATTCTCATGGCCCTGCTGCACGTGGGAGCCCTGTACGGGATCACACTGGTTC
  CCTCCTGCAAGCTCTACACCTGCCTCTTCGCGTTTGTCTACTATGTGATCAGTATTGAGGGCATTGGAGC
  CGGAGTCCATCGCCTGTGGAGCCACAGAACGTACAAGGCACGCCTGCCCCTGCGGATCTTCCTCATCATT
  GCCAACACCATGGCGTTCCAGAATGACGTGTATGAATGGGCCCGAGATCACCGAGCCCACCACAAGTTCT
  CAGAAACACACGCCGACCCTCACAATTCCCGCCGTGGCTTCTTCTTCTCTCACGTGGGTTGGCTGCTTGT
  GCGCAAACACCCGGCTGTCAAAGAGAAGGGCGGAAAACTGGACATGTCTGACCTGAAAGCCGAGAAGCTG
  GTGATGTTCCAGAGGAGGTACTACAAGCCTGGCATTCTGCTGATGTGCTTCATCCTGCCCACGCTGGTGC
  CCTGGTACTGCTGGGGCGAGACTTTTCTAAACAGTTTTTATGTTGCCACTTTACTGAGATACGCTGTGGT
  GCTCAACGCCACTTGGCTGGTGAACAGTGCCGCCCACCTCTACGGGTATCGCCCCTACGATAAGAACATC
  GATCCCCGGCAGAATGCCCTGGTTTCCTTGGGAAGTATGGGCGAGGGCTTCCACAACTACCACCATGCCT
  TCCCCTACGACTACTCTGCCAGTGAGTACCGCTGGCACATCAACTTCACCACGTTCTTCATCGACTGCAT
  GGCTGCACTGGGCCTGGCTTACGACCGGAAGAGAGTGTCCAAGGCCACTGTCTTAGCCAGGATTAAGAGA
  ACTGGAGACGGGAGTCACAAGAGTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024450
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024450.2, NP_077770.1
• RefSeq Size: 3470 bp
• RefSeq ORF: 1080 bp
• Locus ID: 30049
• UniProt ID: Q99PL7
• Cytogenetics: 19 C3
• Gene Summary: Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA (PubMed:16443825, PubMed:26098370). Has a strong preference for saturated fatty acids with chain lengths of 14 or 16 carbon atoms (C14:0 and C16:0), and has only very low activity with stearatate (C18:0) (PubMed:16443825, PubMed:26098370). Required for the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity).[UniProtKB/Swiss-Prot Function]