Pmm1 (NM_013872) Mouse Untagged Clone

CAT#: MC209823

Pmm1 (untagged) - Mouse phosphomannomutase 1 (Pmm1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pmm1
• Synonyms: C77612
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209823 representing NM_013872
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTCGCCGTCGAGGGCGCCCGCAGGAAAGAGCGCATCCTCTGCCTGTTTGACGTGGACGGGACCC
  TCACGCCAGCTCGCCAGAAAATTGACCCTGAGGTATCGGCCTTCCTGCAGAAGCTACGAAGCAGGGTACA
  GATCGGCGTGGTGGGTGGCTCTGACTACTCTAAGATCGCCGAGCAGCTGGGAGAGGGGGATGAAGTCATT
  GAGAAGTTTGACTACGTGTTTGCTGAGAATGGGACAGTGCAGTATAAACACGGACGGCTACTCTCCAAAC
  AGACGATCCAGAACCACCTGGGGGAGGAGCTCCTGCAGGACTTGATCAACTTCTGCCTTAGCTACATGGC
  CCTGCTCAGACTGCCCAAGAAGCGTGGGACCTTCATTGAGTTCCGGAATGGCATGCTGAACGTCTCGCCC
  ATTGGCCGCAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCGGAACTGGACAAGAAGGAGAAGATCCGGG
  AGAAGTTTGTGGAAGCCTTGAAGACAGAGTTTGCTGGCAAGGGGCTGCGGTTCTCCCGAGGAGGCATGAT
  AAGCTTCGATGTCTTCCCCGAGGGCTGGGATAAGCGCTACTGCCTGGACAGCCTGGATGAAGACAGCTTT
  GACATCATCCACTTCTTTGGAAATGAGACCAGTCCTGGCGGGAATGACTTTGAGATCTATGCGGACCCCC
  GGACTGTCGGCCATAGCGTGGTCTCCCCTCAGGACACTGTACAGCGATGCCGTGAGCTCTTCTTCCCAGA
  GACAGCCCACGAGGCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013872
• Insert Size: 789 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013872.4, NP_038900.1
• RefSeq Size: 1316 bp
• RefSeq ORF: 789 bp
• Locus ID: 29858
• UniProt ID: O35621
• Cytogenetics: 15 E1
• Gene Summary: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).