Skp2 (NM_013787) Mouse Untagged Clone

CAT#: MC209793

Skp2 (untagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skp2
• Synonyms: 4930500A04Rik; FBXL1; FWD1; p45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209793 representing NM_013787
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATAGGAAGCACCTTCAGGAGATTCCGGACCAGAGTGGCAACGTCACCACCAGCTTCACGTGGGGAT
  GGGATTCCAGCAAGACTTCTGAACTGCTATCAGGCATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGA
  CAGTGAGAACATCCCACATGGACTGCTCTCAAACCTCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTC
  AAGGGCAAAGGGAGTGACAAAGACTTTGTGATCATCCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAG
  GTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTCCTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCT
  GCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACCGCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTC
  GACCTCGCGGGTAAAAATCTGCACCCAGACGTGACTGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCC
  GCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGTGAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGA
  CCTGTCGAACTCAGTGATAAATGTGTCGAACCTCCATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAAT
  CTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCATTGTCAAGACTCTTGCACAGAATGAAAACTTGGTGC
  GACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAATCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAG
  ACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACTTCACTGAAAAGCACGTGCAAGCGGCTGTGGCACAT
  TTACCAAACACCATCACCCAGCTGAACCTCAGCGGCTACCGAAAGAACCTCCAGAAAACAGATCTTTGTA
  CCATAATTAAACGATGCCCCAACCTCATCCGCCTCGACTTAAGTGACAGTATCATGCTAAAGAATGACTG
  CTTTCCAGAATTTTTTCAACTCAACTACCTCCAACACCTCTCGCTCAGCCGGTGCTATGATATAATACCT
  GATACTCTACTTGAACTTGGAGAAATTCCTACACTAAAAACGCTACAAGTTTTTGGAATCGTGCCAGAGG
  GAACCCTTCAGCTACTGAGGGAAGCTCTTCCTCGGCTGCAGATTAACTGCGCCTATTTCACCACCATTGC
  AAGGCCAACTATGGACAGCAAGAAGAACCTGGAGATTTGGGGTATCAAGTGCCGACTGACTCTGCAAAAG
  CCCAGTTGTCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013787
• Insert Size: 1275 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013787.3, NP_038815.1
• RefSeq Size: 3210 bp
• RefSeq ORF: 1275 bp
• Locus ID: 27401
• UniProt ID: Q9Z0Z3
• Cytogenetics: 15 A1
• Gene Summary: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).