Hsd17b6 (NM_013786) Mouse Untagged Clone

CAT#: MC209792

Hsd17b6 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 6 (Hsd17b6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsd17b6
• Synonyms: 17betaHSD9; Hsd17b9; Rdh8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209792 representing NM_013786
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTTCTACCTGGTAACTCTTGTGGGCCTTTACCACCTTCTGCGTTGGTATCGTGAGAGGCAGGTGG
  TGAGCCATCTCCAAGACAAGTATGTCTTCATCACGGGCTGTGACTCTGGCTTTGGGAACCTGCTGGCCAG
  ACAGCTGGACAGGAGAGGCATGAGGGTATTGGCTGCATGTCTGACGGAGAAGGGAGCCGAGGAGCTGAGG
  AACAAGACATCTGACAGGCTGGAGACAGTGATCCTGGATGTCACCAAGACAGAGAGTATTGTGGCAGCCA
  CTCAGTGGGTGAAGGAGCGTGTTGGAGACAGAGGACTCTGGGGTTTGGTTAATAATGCAGGTGTGTTACA
  ACCATTTGCCTACATTGAATGGTACAGACCAGAGGACTACATGCCTATCTTTCAAGTGAACCTCATTGGT
  TTGACCCAGGTGACTATAAGCATGCTTTTCCTGGTAAAGAAGGCTCGGGGCAGGATCGTCAATGTCTCCA
  GTGCTTTGGGAAGAGTTGCATTGTTTGGAGGATTCTACAGTTGCTCCAAGTATGGGGTTGAGGCATTTTC
  AGATGTGCTAAGGCATGAGGTTCAAGATTTCGGGGTGAAAGTCAGCATAATTGAACCTGGGAGCTTCAAG
  ACGGAAATGACAGATGCAGAGTTAACCATTGAGAGAACTAAGAAAGTCTGGGAAGCTGCCCCTGAGCACA
  TCAAGGAATCCTACGGACAGCAGTTTTTTGACGACTTTTGCAGCACCACCAAACGAGAGTTGATGAAGTG
  TAGCAGGAACCTGAGCCTAGTCACGGACTGCATGGAGCACGCCCTGACCTCCACGCATCCTCGCACCCGG
  TACTCGGCTGGCTGGGATGCCAAGTTTTTCTTCATCCCTCTATCTTATTTGCCTGCGTCACTGGTAGACT
  ACTTATTGGCCATATCTAGGGGCAAGCCAGCTCAAGCAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013786
• Insert Size: 954 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021836, AAH21836
• RefSeq Size: 1479 bp
• RefSeq ORF: 954 bp
• Locus ID: 27400
• UniProt ID: Q9R092
• Cytogenetics: 10 D3
• Gene Summary: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro).[UniProtKB/Swiss-Prot Function]