Map2k2 (NM_023138) Mouse Untagged Clone

CAT#: MC209716

Map2k2 (untagged) - Mouse mitogen-activated protein kinase kinase 2 (Map2k2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map2k2
• Synonyms: AA589381; MEK2; MK2; Prkmk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209716 representing NM_023138
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCCCGGAGGAAGCCGGTGTTACCGGCACTCACTATCAACCCTACCATCGCCGAGGGCCCGTCCC
  CCACCAGCGAGGGCGCCTCTGAGGCAAACCTGGTGGACCTCCAGAAGAAGCTGGAAGAGCTGGACCTGGA
  TGAGCAGCAAAGGAAGCGGCTGGAGGCCTTCCTTACCCAGAAGGCTAAGGTCGGTGAGCTCAAGGACGAC
  GACTTTGAGAGGATCTCAGAGCTGGGTGCCGGCAATGGCGGAGTGGTCACCAAGGCCCGGCATAGGCCGT
  CAGGCCTCATCATGGCCAGAAAGCTGATCCACCTGGAGATCAAACCGGCCGTGCGCAACCAGATCATCCG
  GGAGCTGCAGGTGCTGCACGAGTGCAACTCGCCCTACATCGTGGGCTTCTATGGGGCCTTCTACAGCGAC
  GGCGAGATCAGCATCTGCATGGAGCACATGGATGGTGGCTCACTGGACCAGGTACTGAAGGAGGCCAAGC
  GGATTCCTGAAGACATCTTGGGGAAGGTCAGCATTGCGGTGCTCCGGGGCCTGGCCTACCTCCGAGAGAA
  GCACCAGATCATGCACAGAGATGTGAAGCCCTCCAACATCCTGGTGAACTCTCGCGGGGAGATTAAGCTG
  TGTGACTTCGGGGTGAGCGGCCAGCTGATCGACTCCATGGCCAACTCGTTTGTAGGGACGCGCTCCTACA
  TGTCCCCAGAGCGGCTGCAGGGCACCCACTACTCTGTGCAGTCGGACATCTGGAGCATGGGGCTGTCGCT
  GGTGGAGCTGGCCATCGGGAGGTATCCCATTCCCCCACCTGATGCCAAGGAACTAGAGGCCAGCTTTGGC
  CGGCCTGTGGTGGACGGGGCAGACGGAGAACCCCATAGTGTCTCCCCGAGGCCCAGGCCCCCTGGACGCC
  CCATCAGTGTAGGTCATGGGATGGACAGCCGACCGGCCATGGCCATCTTTGAGCTGCTGGACTACATAGT
  GAACGAGCCACCTCCCAAGCTGCCCAGTGGTGTGTTCAGCTCAGACTTCCAGGAGTTTGTGAATAAATGT
  CTCATTAAGAACCCAGCAGAGCGAGCAGATCTGAAGCTGCTGATGAACCACGCCTTCATCAAGCGCTCTG
  AGGGGGAGGAAGTGGACTTCGCCGGCTGGCTGTGCAGAACCCTGCGGCTGAAGCAGCCCAGCACACCCAC
  GCGGACTGCAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023138
• Insert Size: 1206 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023138.4, NP_075627.2
• RefSeq Size: 2387 bp
• RefSeq ORF: 1206 bp
• Locus ID: 26396
• UniProt ID: Q63932
• Cytogenetics: 10 39.72 cM
• Gene Summary: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).