Wnt3 (NM_009521) Mouse Untagged Clone

CAT#: MC209651

Wnt3 (untagged) - Mouse wingless-related MMTV integration site 3 (Wnt3), (10ug)


  "NM_009521" in other vectors (4)

Reconstitution Protocol

USD 503.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Wnt3
Synonyms Int-4; Wnt-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209651 representing NM_009521
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCCACCTGCTCGGGCTGCTACTCGGCCTCCTGCTCAGTGGCACCAGGGTCCTCGCTGGCTACC
CAATTTGGTGGTCCCTGGCCCTGGGCCAGCAGTACACATCTCTGGCCTCCCAGCCTCTGCTCTGCGGCTC
CATCCCAGGCCTGGTCCCCAAGCAACTGCGCTTCTGCCGCAATTACATCGAGATCATGCCCAGCGTAGCA
GAAGGTGTGAAGCTGGGCATCCAGGAGTGCCAGCATCAGTTCCGGGGCCGCCGGTGGAACTGTACCACCA
TAGATGACAGCCTGGCCATCTTTGGGCCTGTCTTGGACAAAGCCACCCGTGAATCGGCCTTCGTGCATGC
CATCGCCTCGGCTGGTGTCGCCTTCGCAGTCACACGCTCCTGCGCTGAGGGAACCTCCACCATCTGCGGC
TGTGACTCACATCATAAGGGGCCACCTGGAGAAGGCTGGAAGTGGGGCGGCTGCAGCGAGGACGCCGACT
TCGGGGTGCTGGTGTCCCGGGAATTTGCGGATGCGCGGGAGAACAGGCCAGATGCCCGCTCAGCTATGAA
CAAGCACAACAATGAAGCAGGCCGAACGACCATCCTGGACCACATGCACCTAAAGTGTAAATGCCACGGG
TTGTCCGGCAGCTGCGAGGTGAAGACCTGCTGGTGGGCCCAGCCCGACTTCCGTGCCATTGGCGACTTCC
TCAAGGACAAGTACGACAGTGCCTCCGAGATGGTGGTGGAGAAACACCGTGAGTCCCGAGGCTGGGTGGA
GACCCTGCGGGCTAAGTACGCGCTCTTCAAGCCACCCACCGAGAGGGACCTGGTCTACTACGAGAACTCC
CCCAACTTTTGTGAGCCCAACCCAGAGACGGGCTCCTTTGGTACCAGGGACCGGACTTGCAATGTCACCT
CCCACGGCATCGATGGCTGCGATCTGCTGTGCTGTGGCCGGGGCCACAACACGAGGACGGAGAAACGGAA
GGAGAAATGCCATTGCGTCTTCCACTGGTGCTGCTATGTCAGCTGCCAAGAGTGTATTCGCATCTACGAT
GTGCACACCTGCAAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009521
Insert Size 1068 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009521.2, NP_033547.1
RefSeq Size 3115 bp
RefSeq ORF 1068 bp
Locus ID 22415
UniProt ID P17553
Cytogenetics 11 67.5 cM
Gene Summary Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (By similarity). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PubMed:10431240). Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development (PubMed:12569130).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.