Ube2e1 (NM_009455) Mouse Untagged Clone

CAT#: MC209608

Ube2e1 (untagged) - Mouse ubiquitin-conjugating enzyme E2E 1, UBC4/5 homolog (yeast) (Ube2e1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ube2e1
• Synonyms: Ubce5; ubcM2; UbcM3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209608 representing NM_009455
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGATGACGATTCGAGGGCCAGCACCAGCTCCTCGTCATCTTCGTCCTCCAACCAGCAGACCGAGA
  AAGAAGGCAGCACCCCCAAGAAGAAGGAGAGTAAAGTCAGCATGAGCAAAAATTCGAAGCTTCTCTCCAC
  CAGCGCTAAGAGGATCCAGAAGGAGCTGGCAGACATCACTTTAGACCCTCCGCCAAACTGCAGTGCTGGT
  CCCAAGGGTGACAACATCTATGAGTGGAGATCAACCATTCTGGGTCCTCCAGGGTCTGTGTATGAAGGTG
  GAGTATTCTTCCTTGACATTACTTTTACACCAGAGTATCCTTTCAAGCCTCCAAAGGTTACATTTCGTAC
  AAGAATTTATCACTGTAATATTAATAGCCAAGGAGTTATTTGCTTGGACATATTGAAAGACAACTGGAGC
  CCAGCCCTAACCATCTCGAAAGTCCTCCTTTCTATCTGCTCACTCCTTACAGACTGCAATCCTGCTGACC
  CTTTGGTGGGAAGTATTGCCACTCAGTACATGACCAACAGAGCAGAACACGACAGAATGGCCAGACAGTG
  GACCAAGAGATACGCTACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009455
• Insert Size: 582 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009455.3, NP_033481.1
• RefSeq Size: 1386 bp
• RefSeq ORF: 582 bp
• Locus ID: 22194
• UniProt ID: P52482
• Cytogenetics: 14 A2
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]