Tnnt2 (NM_001130174) Mouse Untagged Clone

CAT#: MC209563

Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209563 representing NM_001130174
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGTGGAAGAGGAAGACT
  GGAGTGAAGAAGAGGAGGACGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGA
  GCCTGAGGGTGAGGCTGAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGAT
  GCTGAAGAAGGTCCAGTAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCAC
  CCAAGATCCCCGATGGAGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAA
  TGAGCTACAGACTCTGATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTC
  AAAGACAGGATCGAAAAGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGG
  AAAGGCAGAACCGCCTGGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGA
  TGAGGCCCGGAAGAAGAAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCCAGAAGACAGAGCGG
  AAGAGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGG
  CCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCT
  GGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAAC
  CGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130174
• Insert Size: 909 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130174.2, NP_001123646.1
• RefSeq Size: 1232 bp
• RefSeq ORF: 909 bp
• Locus ID: 21956
• Cytogenetics: 1 59.32 cM
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.