Wnt8a (NM_009290) Mouse Untagged Clone

CAT#: MC209475

Wnt8a (untagged) - Mouse wingless-related MMTV integration site 8A (Wnt8a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnt8a
• Synonyms: Stra11; Wnt-8A; Wnt-8D; Wnt8d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209475 representing NM_009290
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACACTTGTTAATGCTGTGGGTGGCTGCGGGCATGTGCTATCCAGCCCTGGGTGCTTCTGCCTGGT
  CAGTGAACAACTTCCTGATAACCGGTCCCAAGGCCTATCTGACCTACACCGCCAGTGTGGCCTTGGGAGC
  TCAGATTGGCATCGAAGAGTGTAAGTTCCAGTTTGCCTGGGAACGGTGGAATTGTCCTGAGCATGCTTTT
  CAGTTTTCAACCCACAACAGGCTGCGAGCTGCCACGAGAGAGACATCCTTCATTCATGCCATCCGCTCTG
  CTGCCATCATGTACGCAGTCACCAAGAACTGCAGCATGGGTGACTTGGAAAACTGCGGCTGTGACGAGTC
  ACAAAATGGAAAAACAGGTGGCCATGGCTGGATCTGGGGAGGCTGCAGCGACAACGTGGAGTTCGGGGAA
  AAAATCTCCAGACTCTTCGTGGACAGTTTGGAGAAAGGGAAGGATGCCAGAGCCCTGGTGAACCTTCACA
  ACAACAGGGCCGGCAGACTGGCAGTGAGGGCCTCCACGAAAAGGACCTGCAAGTGTCATGGCATCTCAGG
  AAGCTGCAGCATCCAGACGTGTTGGCTGCAGCTGGCTGACTTCCGGCAGATGGGAAATTACCTAAAGGCC
  AAGTATGACCGCGCGCTGAAAATTGAGATGGACAAGCGCCAGCTAAGGGCTGGCAACAGAGCCGAGGGCC
  GCTGGGCTCTCACGGAGGCCTTCCTTCCCAGCACAGAGGCTGAGCTGATCTTCTTAGAGGGGTCTCCTGA
  CTACTGCAACCGCAACGCCAGCCTGAGCATCCAGGGCACAGAGGGGAGGGAGTGCCTGCAGAATGCCCGC
  AGTGCTTCCCGGCGGGAGCAGCGCAGCTGTGGGCGCCTGTGCACGGAGTGCGGGCTGCAGGTGGAGGAGA
  GGAGAGCAGAGGCCGTGAGCAGCTGTGACTGCAACTTTCAGTGGTGTTGCACTGTCAAGTGTGGCCAGTG
  CAGGCGTGTGGTGAGCAGATACTACTGCACACGCCCTGTAGGTAGTGCCAGGCCCCGGGGCAGGGGCAAG
  GACAGTGCCTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009290
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009290.2, NP_033316.1
• RefSeq Size: 1519 bp
• RefSeq ORF: 1065 bp
• Locus ID: 20890
• UniProt ID: Q64527
• Cytogenetics: 18 B1
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.[UniProtKB/Swiss-Prot Function]