Ssb (NM_009278) Mouse Untagged Clone

CAT#: MC209465

Ssb (untagged) - Mouse Sjogren syndrome antigen B (Ssb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ssb
• Synonyms: SS-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209465 representing NM_009278
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAAATGGAGATAATGAAAAAATGACTGCTTTGGAGGCCAAAATCTGTCATCAAATTGAGTATT
  ATTTTGGAGACTTCAATTTGCCACGAGACAAGTTTTTAAAAGAACAGATCAAATTGGATGAAGGCTGGGT
  ACCTTTGGAAACAATGATAAAATTCAACAGGCTAAACCGGCTGACAACAGACTTTAATGTAATTGTGCAA
  GCACTGAGCAAATCTAAGGCAAAACTCATGGAAGTCAGTGCAGACAAAACTAAAATTAGAAGATCACCAA
  GCAGACCACTCCCTGAAGTGACGGATGAGTATAAGAATGATGTAAAAAACAGATCTGTTTATATTAAAGG
  TTTCCCAACTGACGCCACCCTTGATGATATAAAAGAATGGCTAGACGATAAAGGCCAAATACTGAATATT
  CAAATGAGAAGAACATTACACAAAACATTTAAGGGGTCAATATTTGCTGTGTTTGATAGTATTCAGTCTG
  CAAAGAAGTTTGTGGAGATCCCTGGCCAGAAGTACAAAGACACTAACCTGCTAATACTCTTTAAGGAAGA
  TTACTTTGCAAAAAAAAATGAAGAAAGAAAGCAGAGCAAAGTGGAAGCTAAATTAAAAGCTAAACAAGAG
  CATGAAGGAAGACACAAGCCAGGAAGTACTGAAACCAGAGCTCTAGAAGGAAAGATGGGATGCTTACTGA
  AGTTTTCAGGTGACTTGGATGACCAGACCTGTAGAGAAGATTTACACTTCCTTTTCTCAAATCATGGTGA
  AATAAAATGGGTCGACTTTGCCAGAGGAGCAAAAGAGGGAATAATTCTCTTCAAAGAAAAGGCTAAGGAA
  GCACTTGAGAAAGCCAGAAATGCAAATAATGGTAACCTACTGTTAAGGAACAAAAAGGTGACTTGGAAAG
  TACTAGAAGGACATGCGGAAAAAGAAGCATTGAAAAAAATCACAGATGATCAGCAAGAATCCCTAAACAA
  ATGGAAGTCAAAAGGAGGGCATGCAGGTGGAAGATTTAAAGGAAGCCATGTTTTTACAGCAGCCCGCAGA
  TTTAAAGGAAAAGGGAAGGGTAATAGGCCTGGTTATGCTGGGGCACCCAAAGGAAGAGGACAGTTTCATG
  GAAGGAGGACAAGATTTGATGATGATGATCGTCGTCGTGGACCAATGAAAAGAGGAAGAGATGGAAGAGA
  CAGAGAAGAACCCGCATCAAAGCATAAGAAAAGAGAAAATGGTGCTAGAGACAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009278
• Insert Size: 1248 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009278.4, NP_033304.1
• RefSeq Size: 2088 bp
• RefSeq ORF: 1248 bp
• Locus ID: 20823
• UniProt ID: P32067
• Cytogenetics: 2 40.95 cM
• Gene Summary: Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.