Sprr2f (NM_011472) Mouse Untagged Clone

CAT#: MC209458

Sprr2f (untagged) - Mouse small proline-rich protein 2F (Sprr2f), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sprr2f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209458 representing NM_011472
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTACCAAGAACAGCAGTGCAAGCAACCCTGCCAGCCTCCTCCTGTGTGTCCACCACCAAAGTGCC
  CAGAGCCTTGTTCTCCCTCGGTGTGCCCTGAGCCTTGTCCTCCTCCAAAGTGCCCTGAGCCTTGTCCTGA
  GCCATGTCCCCCTCCCTCATTCCAGCAGAAATGCCCTCCTGTGCAACCTCCTCCACCCTGCCAGCAGAAG
  TGCCCACCCAAGAGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011472
• Insert Size: 231 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011472.2, NP_035602.1
• RefSeq Size: 609 bp
• RefSeq ORF: 231 bp
• Locus ID: 20760
• UniProt ID: O70557
• Cytogenetics: 3 40.14 cM
• Gene Summary: Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane (By similarity).[UniProtKB/Swiss-Prot Function]