Spic (NM_011461) Mouse Untagged Clone

CAT#: MC209446

Spic (untagged) - Mouse Spi-C transcription factor (Spi-1/PU.1 related) (Spic), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spic
• Synonyms: AU019198; C76795; Prf; Spi-C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209446 representing NM_011461
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTGTTGTATTGATCAAGACAGTTTGGGGCAAACATTTCAAGACGCCATTGACATATTGATCCAAC
  AATCAGCTGGAGAAAGTCAATACTCCTCAGAAAATAGAAATTACATGGCTATCATTAACCCTTATCCTCA
  CGTCAGAGGCAACGCTAACTACTATGGAATGTCACCCACAGAGAACCCCCTCTATGACTGGAGAGGTGTA
  ACAAATGGTTCTGCAGACCTGTATTTGGAAGGAGGCTTCCACCAATCCGTACAGAACATAGCTGAAAGCC
  AGCTGGTACAACCCCCCTTCTTCCAGCAAAAGGGAGGAAGAGGCAGGAGAAAGCTTCGGCTGTTTGAATA
  CCTTTTCGAATCCCTGTGCAATTCAGAGATGGTGTCTTGTATTCAGTGGGTTGACAAAGCCAGAGGCATC
  TTTCAGTTTATATCAAAAAACAAAGAAACACTCGCTGAACTCTGGGGGCAGAGAAAAGGCAACCGGAAAC
  CCATGACTTACCAGAAGATGGCCAGAGCCCTGAGGAATTATGCCAGAACTGGGGAGATCATAAAAATCCG
  GAGAAAGCTGACCTACCAGTTCAGCGAGGCTGTTCTCCAAAGACTTGCTCCAGCTAACTACCTGGGAAAA
  GACCTCTTCTACCCTCAGTATGGCCAACCTGATCAGGGCTACCTCAGTTTAAACCACTGGAATGCAAATC
  ACTATGCTCACGGCAGTTACCAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011461
• Insert Size: 729 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC064038, AAH64038
• RefSeq Size: 1266 bp
• RefSeq ORF: 729 bp
• Locus ID: 20728
• UniProt ID: Q6P3D7
• Cytogenetics: 10 C1
• Gene Summary: Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression.[UniProtKB/Swiss-Prot Function]