Sphk1 (NM_001172473) Mouse Untagged Clone

CAT#: MC209437

Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sphk1
• Synonyms: 1110006G24Rik; SK; Sk1; Spk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209437 representing NM_001172473
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGCTGGTGCTGCTGAACCCCC
  AGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTTCCTGGAGGAGGCAGAGAT
  AACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTGTGTGCAGAGGAGTTGGGT
  CACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGGTGAATGGGCTAATGGAAC
  GGCCAGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGGCTCCGGCAATGCGCTGGC
  AGCTTCTGTGAACCACTATGCTGGGTACGAGCAGGTGACTAATGAAGACCTGCTCATCAACTGCACACTG
  CTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTGCTTCTGGGCTGCGGCTCT
  ATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAGTGAGAAGTACAGGCGCTT
  GGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGCATCTACCAAGGCCAACTG
  GCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGGTGCAGAAGGGCCCCGTCG
  ACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGTACCAGAACAGGACTTCGT
  CCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCACCCATGGGCCGCTGTGAG
  GCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGCTGCTGCGCCTCTTCCTGG
  CCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGTGCCCGTGGTTGCTTTCCG
  CCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATGGTATGTGAAGCTGTGCAG
  GGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCCCATCCGGCCGGGACTCCC
  GGCGGGGGCCACCTCCAGAAGAACCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172473
• Insert Size: 1149 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172473.1, NP_001165944.1
• RefSeq Size: 1766 bp
• RefSeq ORF: 1149 bp
• Locus ID: 20698
• UniProt ID: Q8CI15
• Cytogenetics: 11 E2
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (3) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.