Sox15 (NM_009235) Mouse Untagged Clone

CAT#: MC209432

Sox15 (untagged) - Mouse SRY-box containing gene 15 (Sox15), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sox15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209432 representing NM_009235
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGACCAGCTCCTCACAAGCAGAGACTTGGAGCCTGCATCCTCGGGCTTCCACGGCCTCTTTGC
  CTTTAGGACCCCAGGAGCAGGAGGCCGGCGGGAGCCCTGGAGCGTCTGGGGGACTTCCGCTGGAGAAGGT
  GAAGCGGCCCATGAACGCCTTCATGGTGTGGAGCTCTGTTCAGCGCCGCCAGATGGCGCAGCAGAACCCC
  AAGATGCACAACTCTGAGATCTCCAAGCGCTTGGGCGCTCAGTGGAAGCTGCTGGGCGATGAAGAGAAGC
  GACCCTTCGTGGAGGAGGCTAAGCGTCTTCGTGCCCGCCACCTCCGCGACTATCCCGACTACAAGTACCG
  ACCCCGGCGTAAGAGCAAAAACTCGAGCACCGGGTCTGTCCCCTTTAGCCAAGAAGGAGGTGGCCTGGCA
  TGTGGTGGCTCACACTGGGGGCCAGGGTACACAACTACCCAAGGGAGCAGAGGCTTTGGGTACCAGCCCC
  CCAACTATTCGACAGCCTACCTGCCTGGCAGTTACACCTCTTCCCACTGCAGACCGGAGGCCCCCTTACC
  ATGCACCTTCCCTCAGAGTGATCCCAGGCTCCAGGGGGAGCTAAGACCCTCTTTCTCCCCTTACCTATCC
  CCAGACTCTTCCACTCCATATAATACTTCCCTTGCTGGAGCCCCCATGCCAGTAACCCACCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009235
• Insert Size: 696 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009235.2, NP_033261.1
• RefSeq Size: 1156 bp
• RefSeq ORF: 696 bp
• Locus ID: 20670
• UniProt ID: P43267
• Cytogenetics: 11 42.86 cM
• Gene Summary: Transcription factor that binds to DNA at the 5'-AACAATG-3' consensus sequence (PubMed:10821863, PubMed:15863505, PubMed:16759287, PubMed:17363903). Acts as a transcriptional activator and repressor (PubMed:10821863, PubMed:15863505, PubMed:16759287). Binds synergistically with POU5F1 (OCT3/4) to gene promoters (PubMed:15863505). Binds to the FOXK1 promoter and recruits FHL3, resulting in transcriptional activation of FOXK1 which leads to myoblast proliferation (PubMed:17363903). Acts as an inhibitor of myoblast differentiation via transcriptional repression which leads to down-regulation of the muscle-specific genes MYOD and MYOG (PubMed:10821863). Involved in trophoblast giant cell differentiation via enhancement of HAND1 transcriptional activity (PubMed:16759287). Regulates transcription of HRC via binding to it proximal enhancer region (PubMed:15863505). Involved in skeletal muscle regeneration (PubMed:15367664, PubMed:17363903). Also plays a role in the development of myogenic precursor cells (PubMed:15367664).[UniProtKB/Swiss-Prot Function]