Sla (NM_009192) Mouse Untagged Clone

CAT#: MC209410

Sla (untagged) - Mouse src-like adaptor (Sla), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sla
• Synonyms: Slap; Slap-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209410 representing NM_009192
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAATAGCATGAAATCCACATCTCCACCTTCGGAGAGGCCGTTATCCAGCAGTGAGGGACTTGAAA
  GTGACTTCCTGGCTGTGCTGACTGACTACCCATCTCCTGACATTAGCCCCCCAATATTCCGGAGAGGAGA
  GAAACTGCGTGTAATTTCTGATGAAGGGGGCTGGTGGAAAGCCATTTCTCTCAGCACTGGCCGGGAAAGT
  TATATTCCAGGGATATGTGTGGCCAGAGTGTACCATGGCTGGCTGTTTGAAGGACTGGGCAGGGACAAGG
  CTGAGGAACTGCTGCAGCTGCCAGATACAAAGATTGGTAGCTTCATGATTCGAGAGAGTGAAACAAAGAA
  AGGTTTCTATTCGCTGTCTGTGAGACACAGGCAGGTGAAGCATTATCGAATCTTCCGTCTTCCCAACAAC
  TGGTACTACATCTCACCAAGGCTCACCTTCCAATGCCTGGAGGACCTGGTGACTCATTATTCTGAAGTGG
  CTGATGGTCTATGCTGTGTGCTCACCACACCCTGTCTGGCACAGAACATACCTGCTCCAACATCCCACCC
  ATCACCTTGCACCAGCCCTGGTTCACCTGTCACCTTGCGCCAGAAGACCTTTGACTGGAAGAGGGTATCC
  AGATTGCAAGAAGGTTCAGAGGGAGCAGAGAACCCACTCAGAGTGGACGAATCCCTTTTCAGCTATGGTC
  TTCGGGAAAGCATTGCCTCTTACCTGTCCCTGACAGGGGATGACAGCAGCTCCTTCGACCGGAAGAAGAA
  GAGTCTCTCCCTAATGTACACTGGCAGCAAACGCAAGAGTTCCTTTTTTTCAGCACCCCAGTACTTTGAA
  GATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009192
• Insert Size: 846 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009192.3, NP_033218.1
• RefSeq Size: 3135 bp
• RefSeq ORF: 846 bp
• Locus ID: 20491
• UniProt ID: Q60898
• Cytogenetics: 15 29.3 cM
• Gene Summary: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.