Sgca (NM_009161) Mouse Untagged Clone

CAT#: MC209386

Sgca (untagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sgca
• Synonyms: 50DA; 50DAG; A; ad; Asg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209386 representing NM_009161
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGCAGTAACTTGGATACCTCTCCTGGCAGGTCTCCTGGCAGGACTGAGGGACACCAAGGCCC
  AGCAGACAACTTTACACCTACTTGTGGGTCGTGTGTTTGTGCATCCTTTGGAACATGCCACCTTCCTGCG
  CCTTCCAGAACACGTTGCGGTGCCACCCACTGTCCGACTCACCTACCACGCTCACCTCCAGGGACATCCA
  GACCTGCCCAGGTGGCTGCACTACACACAGCGCAGTCCCTATAACCCTGGCTTCCTCTACGGCTCCCCCA
  CTCCAGAAGATCGTGGGTACCAAGTCATCGAGGTCACAGCCTACAATCGAGACAGTTTTGACACCACTAG
  ACAGAGGCTGCTGCTGCTGATTGGGGACCCCGAAGGTCCCCGGTTGCCATACCAAGCTGAGTTCCTGGTG
  CGCAGCCATGATGTGGAGGAGGTGCTGCCCACCACACCTGCCAACCGCTTCCTCACCGCCTTGGGGGGAC
  TGTGGGAGCCAGGAGAGCTTCAGCTGCTCAACATCACTTCCGCCTTGGACCGGGGAGGCCGAGTCCCTCT
  TCCTATTGAGGGACGGAAGGAAGGGGTATACATTAAGGTAGGCTCTGCCACACCCTTCTCCACCTGCCTG
  AAGATGGTGGCGTCGCCCGACAGCTATGCCCGTTGTGCCCAGGGACAGCCTCCACTACTGTCCTGCTACG
  ACACTTTGGCACCCCACTTCCGCGTTGACTGGTGCAATGTGTCTCTGGTAGACAAGTCAGTACCCGAGCC
  CCTGGATGAGGTACCTACTCCAGGCGATGGGATCTTGGAGCACGACCCGTTCTTCTGCCCACCCACTGAA
  GCCACAGACCGAGACTTCCTGACAGATGCCTTGGTGACCCTCTTGGTGCCTTTGTTGGTGGCTCTGCTGC
  TTACTCTGTTGCTGGCTTACATCATGTGCTTTCGGCGTGAAGGACGGCTGAAGAGAGACATGGCCACCTC
  TGACATCCAGATGTTTCACCACTGTTCCATCCATGGGAATACAGAAGAGCTTCGGCAGATGGCAGCCAGC
  CGAGAGGTGCCCCGGCCTCTTTCCACCTTGCCCATGTTTAATGTTCGTACAGGAGAGCGGTTACCTCCCC
  GAGTAGACAGCGCACAGATGCCTCTTATCCTGGACCAGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009161
• Insert Size: 1164 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009161.4, NP_033187.1
• RefSeq Size: 1738 bp
• RefSeq ORF: 1164 bp
• Locus ID: 20391
• UniProt ID: P82350
• Cytogenetics: 11 D
• Gene Summary: This gene encodes a member of the sarcoglycan alpha/epsilon family of transmembrane proteins. The encoded protein is part of the dystrophin-glycoprotein complex which links the extracellular matrix to the cytoskeleton in muscle fibers. Disruption of this gene results in progressive muscular dystrophy and is associated with the development of embryonal rhabdomysarcoma. [provided by RefSeq, Dec 2012]