Rpgr (NM_001177954) Mouse Untagged Clone

CAT#: MC209322

Rpgr (untagged) - Mouse retinitis pigmentosa GTPase regulator (Rpgr), transcript variant 6, (10ug)


  "NM_001177954" in other vectors (4)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Rpgr
Synonyms Rd9; Rp3h
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209322 representing NM_001177954
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAAGAGGGTCGCGATGGGGGTCCCAGGGGGTAGGGCAGCACCTTAGGCTCAATCGAGTCGCCCCTG
CTATCTTTCCGAAGCAGGCACAGATTCCGTTCGCAGGCTTCGGCATGGCGGAATCTGAGTCACTGGTGCC
CGATACAGGTGCTGTGTTTACGTTTGGAAAAACTAAATTTGCCGAAAATATTCCTAGCAAATTCTGGTTT
AAAAATGACATACCCATATGTCTTTCATGTGGAGATGAACATACTGCTATTGTTACAGGAAATAATAAAT
TGTACATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAAC
ATGTATCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCA
ACAGATACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACG
ATAGAGACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGC
CAATACATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATT
GGTCTAGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCT
CTTGTGGATATTACCATTCAGCTTTTGTAACAAGTAAGATGAACAGTTTTGATTCATTTCCAAGTTATTA
CTGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001177954
Insert Size 777 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001177954.1, NP_001171425.1
RefSeq Size 2507 bp
RefSeq ORF 777 bp
Locus ID 19893
Cytogenetics X A1.1
Gene Summary Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (6) lacks exons in the 3' coding region and differs in the 3' coding region and UTR compared to variant 1. The resulting protein (isoform 6) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.