Rbmy (NM_011253) Mouse Untagged Clone

CAT#: MC209301

Rbmy (untagged) - Mouse RNA binding motif protein, Y chromosome, family 1, member A1 (Rbmy1a1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbmy
• Synonyms: RBM; Rbm1; Rbm1-rs1; Rbmy1a1; Rbmy1a1-rs1; Rbmy1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209301 representing NM_011253
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAACTAATCAGCCTGGGAAAATTTTTATAGGAGGCCTCAACATAAAGACCAGACAAAAGACTC
  TTCAAGAAATATTTGGGAGATTTGGACCAGTAGCACGTGTTATTTTGATGAGAGATCGTGAAACTAAGAA
  GTCTAGAGGCTTTGCTTTCCTTACTTTCCGACGCCTTGCAGATGCTAAGAATGCTGTCAAAGAAATGAAT
  GGAGTGATTTTGGATGGAAAAAGAATTAAAGTAAAACAAGCCAGGAGACCATCATCACTTGAAAGTGGTA
  GCAAGAAGAGACCACCATCCTTTTCAAGAACCAGAGGTGCTTCAAGAATTCTGAAATGTGGAAGAGGAGG
  AAGAAGCAGAGCAAGAAGTGGTCCTTCATGTGAAGGGAACTTGGGTGGTGATAGATATACTCCTAATTTC
  AATGTGAGTTCTTCTGGGAGACACTTTGCAGTTAAAAGAAATCCATCTTCAAAAAGGGATGATCCCCCTT
  CTAAAAGATCTGCAACTTCTGCTCAGACAAGAAGCAATACTGGACTAAGAGGACGAGAACCACATCGAAG
  AGAGATTTCTAGAAATATGCCAAGAGGAGAGCCGGCGTCTTCCAGAAGAGATGAGTATCCATTACCAAGG
  GATTATGGCCAGTCTTCTAATGATAGGAAATATGAATCAACATCCAGAGGATACTGTGACTATGGCAATT
  ACCACTCTCGGGAAGAAAGTGCATCTAAAGTCTTCAGTGATCATGCTGGCTATCTTGGAGGACGTGATAG
  AGATTTCTCTGAATATTTAAGTGGAAATTCATACAGAGATACATACAGAAGTTATGGAAGATTCCATGAG
  GCACCATCTGCAAGAGGTGGAAACAACCGCTATGATGACTATAGTAACTCACAAGATGGATATGGTGGAA
  GAGGAGAACCATACATAAGCAACCGAAGTAACATATACTCAAGTGACTATGAACGTAGTGGTAGACAAGA
  AGTGCTTCCACCACCTATTGATAGGGAATACTTTGACCGTGAGGGCAGACAGGAAAGAGGGCATTCACCT
  AAGGATGGGCTGTATAGTGCATCTCGTGAATCATACAGTAGTAATACCAAGATTTGGGGGATCCCATGGA
  GGTCGTGGAGAAAGCAGATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011253
• Insert Size: 1143 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011253.2, NP_035383.2
• RefSeq Size: 1731 bp
• RefSeq ORF: 1143 bp
• Locus ID: 19657
• UniProt ID: O35698
• Cytogenetics: Ypter
• Gene Summary: RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.