Purb (NM_011221) Mouse Untagged Clone

CAT#: MC209267

Purb (untagged) - Mouse purine rich element binding protein B (Purb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Purb
• Synonyms: 2310015K15Rik; AA114818; Cager-2; D11Bwg0414e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209267 representing NM_011221
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGCGACAGCGGCAGCGAGCGCGGCGGTGGCGGCGGCGGCGGCGGCGGGCCCGGCGGCTTCC
  AGCCCGCGCCCCGCGGCGGCGGCGGCGGCGGTGGCGGGCCGGGCGGCGAGCAGGAGACGCAGGAGCTGGC
  CTCGAAGCGGCTGGACATCCAGAACAAGCGCTTCTACCTGGACGTGAAGCAGAACGCCAAGGGCCGCTTC
  CTCAAGATCGCCGAGGTGGGCGCGGGCGGCTCCAAGAGCCGCCTCACGCTGTCGATGGCGGTGGCCGCCG
  AGTTCCGCGACTCGCTGGGCGACTTCATCGAGCACTACGCGCAGCTGGGTCCCAGCAGCCCCGAGCAGCT
  GGCGGCGGGCGCCGAGGAGGGCGGCGGGCCGCGCCGCGCGCTCAAGAGCGAGTTCCTGGTGCGCGAGAAC
  CGCAAGTACTACCTGGACCTCAAGGAGAACCAGCGCGGCCGCTTCCTGCGCATCCGCCAGACGGTGAACC
  GCGGCGGCGGCGGCTTCGGCGGGGGGCCCGGGCCCGGCGGCCTGCAGAGCGGCCAGACCATCGCGCTGCC
  CGCGCAGGGCCTCATCGAGTTCCGCGACGCGCTCGCCAAGCTCATCGACGACTACGGGGGCGACGAGGAC
  GAGCTGGCCGGCGGCCCGGGAGGCGGCGCCGGCGGCCCCGGGGGCGGCCTGTACGGAGAGCTCCCGGAGG
  GCACCTCCATCACCGTGGACTCGAAGCGCTTCTTCTTCGACGTCGGCTGCAACAAGTACGGGGTGTTCCT
  GCGAGTGAGCGAGGTGAAGCCGTCCTACCGCAATGCCATCACCGTGCCCTTCAAGGCCTGGGGCAAGTTT
  GGAGGCGCCTTCTGTCGGTATGCAGATGAAATGAAAGAGATCCAGGAGCGACAGAGGGATAAGCTTTACG
  AGCGACGCGGCGGGGGCAGCGGTGGCGGCGACGAGTCGGAGGGTGAGGAAGTGGATGAGGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011221
• Insert Size: 975 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011221.3, NP_035351.1
• RefSeq Size: 8478 bp
• RefSeq ORF: 975 bp
• Locus ID: 19291
• UniProt ID: O35295
• Cytogenetics: 11 4.16 cM
• Gene Summary: Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity). Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts.[UniProtKB/Swiss-Prot Function]