Ptger1 (NM_013641) Mouse Untagged Clone

CAT#: MC209255

Ptger1 (untagged) - Mouse prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptger1
• Synonyms: 42kDa; EP1; Ptgerep1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209255 representing NM_013641
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCTGCGGGCTTAACCTGAGCCTAGCGGATGAGGCAGCAACGTGCGCAACACCCAGGCTCCCCA
  ATACATCTGTGGTGCTGCCAACAGGCGATAATGGCACATCACCAGCGCTGCCTATCTTCTCCATGACGCT
  GGGTGCTGTGTCCAACGTGCTGGCGCTGGCGCTGCTGGCCCAGGTTGCAGGCCGAATGCGGCGGCGCCGC
  TCGGCTGCCACCTTCCTGTTGTTCGTCGCCAGCCTGCTTGCCATCGACCTAGCAGGCCATGTGATCCCGG
  GCGCCCTGGTGCTTCGCCTGTATACTGCGGGGCGTGCGCCTGCTGGAGGGGCGTGTCATTTCCTGGGTGG
  CTGCATGGTCTTCTTCGGCCTGTGCCCACTTTTGCTTGGCTGTGGCATGGCCGTAGAGCGCTGTGTGGGT
  GTCACGCAGCCACTGATCCACGCGGCGCGCGTATCTGTGGCCCGCGCACGCCTGGCACTAGCCGTGCTGG
  CTGCCATGGCTTTGGCAGTGGCGCTGCTGCCACTGGTACACGTGGGTCGCTACGAGTTACAGTACCCTGG
  CACCTGGTGTTTTATTAGCCTTGGGCCTCGTGGAGGTTGGCGCCAGGCGTTGCTTGCCGGCCTCTTCGCC
  GGCCTTGGCCTGGCCGCGCTCCTTGCGGCATTAGTGTGCAATACGCTCAGCGGCCTGGCGCTCCTCCGTG
  CCCGCTGGAGACGGCGTCGCTCTCGACGATTCCGAAAGACCGCAGGTCCCGATGATCGCCGGCGCTGGGG
  ATCTCGTGGACCCCGCTTGGCCTCCGCCTCGTCTGCCTCATCCATCACTTCAGCCACAGCCACCCTCCGC
  AGCTCTCGGGGCGGCGGCTCCGCGCGCAGGGTTCACGCACACGATGTGGAAATGGTGGGCCAGCTCGTGG
  GCATCATGGTGGTGTCGTGCATCTGCTGGAGCCCCCTGCTGGTGTTGGTGGTGTTGGCCATCGGGGGCTG
  GAACTCTAACTCCCTGCAGCGGCCACTCTTTCTGGCTGTACGCCTCGCATCGTGGAACCAGATCCTGGAC
  CCATGGGTGTACATCCTGCTGCGCCAGGCCATGCTGCGCCAACTGCTTCGCCTCCTACCCCTGAGGGTCA
  GTGCCAAGGGTGGTCCAACGGAGCTGGGCCTAACCAAGAGTGCCTGGGAAGCCAGTTCACTGCGTAGTTC
  CCGGCACAGTGGTTTCAGTCACCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013641
• Insert Size: 1218 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138739, AAI38740
• RefSeq Size: 1978 bp
• RefSeq ORF: 1218 bp
• Locus ID: 19216
• UniProt ID: P35375
• Cytogenetics: 8 40.22 cM
• Gene Summary: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues.[UniProtKB/Swiss-Prot Function]