Prtn3 (NM_011178) Mouse Untagged Clone

CAT#: MC209240

Prtn3 (untagged) - Mouse proteinase 3 (Prtn3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prtn3
• Synonyms: mPR3; PR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209240 representing NM_011178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAGCTACCCATCCCCCAAGGGGATCCATCCCTTCCTGCTGCTTGCCCTGGTGGTTGGTGGCG
  CAGTCCAGGCCTCCAAGATTGTAGGTGGGCACGAGGCTCGGCCCCACTCTCGGCCTTATGTGGCATCCCT
  GCAGCTGAGCAGGTTCCCTGGGAGCCACTTCTGTGGTGGCACCCTGATCCACCCGAGATTCGTGCTGACA
  GCCGCCCACTGCCTGCAGGACATCTCCTGGCAGCTTGTGACAGTGGTGCTGGGTGCCCACGACCTGCTGA
  GCTCGGAGCCTGAGCAGCAGAAGTTCACCATCAGTCAGGTCTTCCAGAACAATTACAACCCCGAGGAGAA
  CCTCAATGACGTGCTTCTCCTCCAGCTAAACCGGACAGCCTCCCTGGGCAAGGAGGTGGCGGTGGCTTCT
  CTGCCCCAGCAGGACCAGACTCTGTCCCAGGGCACCCAGTGCCTGGCCATGGGCTGGGGTCGCCTGGGCA
  CCCAAGCACCCACGCCCCGTGTGCTGCAGGAACTGAACGTCACGGTGGTCACCTTCCTATGCCGGGAACA
  CAACGTGTGCACGCTGGTGCCACGGAGGGCAGCAGGCATATGCTTCGGAGACTCGGGCGGCCCCTTGATC
  TGCAATGGCATTCTTCATGGAGTGGACTCCTTCGTGATCCGCGAATGCGCCTCCCTCCAGTTCCCTGATT
  TCTTCGCCCGGGTGTCCATGTATGTGGACTGGATTCAAAACGTGCTGCGGGGCGCAGAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011178
• Insert Size: 765 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011178.2, NP_035308.2
• RefSeq Size: 983 bp
• RefSeq ORF: 765 bp
• Locus ID: 19152
• UniProt ID: Q61096
• Cytogenetics: 10 39.72 cM
• Gene Summary: Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.[UniProtKB/Swiss-Prot Function]