Prm2 (NM_008933) Mouse Untagged Clone

CAT#: MC209232

Prm2 (untagged) - Mouse protamine 2 (Prm2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prm2
• Synonyms: AI528784; Prm; Prm-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209232 representing NM_008933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCGCTACCGAATGAGGAGCCCCAGTGAGGGTCCGCACCAGGGGCCTGGACAAGACCATGAACGCG
  AGGAGCAGGGGCAGGGGCAAGGGCTGAGCCCAGAGCGCGTAGAGGACTATGGGAGGACACACAGGGGCCA
  CCACCACCACAGACACAGGCGCTGCTCTCGTAAGAGGCTACATAGGATCCACAAGAGGCGTCGGTCATGC
  AGAAGGCGGAGGAGACACTCCTGCCGCCACAGGAGGCGGCATCGCAGAGGCTGCAGAAGATCCCGAAGGA
  GGAGGAGATGCAGGTGCAGGAAATGTAGGAGGCACCATCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008933
• Insert Size: 324 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008933.2, NP_032959.1
• RefSeq Size: 624 bp
• RefSeq ORF: 324 bp
• Locus ID: 19119
• UniProt ID: P07978
• Cytogenetics: 16 5.84 cM
• Gene Summary: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis, and are the major DNA-binding proteins in the nucleus of sperm in many vertebrates. They package the sperm DNA into a highly condensed complex in a volume less than 5% of a somatic cell nucleus. Many mammalian species have only one protamine (protamine 1); however, a few species, including human and mouse, have two. This gene encodes protamine 2, which is synthesized as a precursor and then cleaved to give rise to a family of protamine 2 peptides. [provided by RefSeq, Sep 2015]