Ppt1 (NM_008917) Mouse Untagged Clone

CAT#: MC209220

Ppt1 (untagged) - Mouse palmitoyl-protein thioesterase 1 (Ppt1), (10ug)


  "NM_008917" in other vectors (4)

Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (2)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ppt1
Synonyms 9530043G02Rik; AA960502; C77813; CLN1; D4Ertd184e; INCL; PPT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209220 representing NM_008917
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGTCCTGTTCGCGGAGGCTGCTAGCTGCCGCTCTGCTGCCTTGGTGCTGCGCCGCCTGGGCCC
TGGGGCATCTGGACCCACCTTCACCACCACCGCTGGTGATCTGGCATGGGATGGGGGACAGCTGTTGTAA
CCCCATGAGCATGGGTGTCATTAAAAAGATGGTTGAAAAAGAAATACCTGGGATTTACGTCCTGTCTCTA
GAGATTGGGAAGAACATGATGGAGGATGTGGAGAACAGCTTCTTCTTGAATGTTAATGTCCAAGTCAACA
TGGTGTGTCAGATTCTGGAAAAGGATCCTAAGTTGCAGCAGGGATACAATGCTATTGGCTTCTCCCAGGG
AGGCCAGTTCCTGAGGGCAGTGGCTCAGAGATGCCCAACACCTCCCATGATGACCCTGATCTCAGTTGGA
GGACAACATCAAGGTGTCTTTGGACTCCCCCGATGCCCAGGAGAGAGTTCTCACATCTGCGACTTCATCA
GGAAGTCACTTAATGCCGGTGCTTACTCCAAACTTGTGCAAGAACGCCTGGTGCAAGCACAGTACTGGCA
TGACCCTATCAAGGAGAGTGTGTACCGAAACTACAGCATCTTCTTGGCAGACATAAATCAAGAGAGGTGT
GTCAATGAGTCCTACAAGAAGAACCTGATGGCCCTCAAGAAGTTTGTGATGGTGAAATTCTTTAATGATT
CCATTGTGGACCCTGTCGACTCTGAGTGGTTTGGATTTTACAGAAGTGGCCAAGCTAAGGAAACCATTCC
CCTCCAGGAGAGCACTCTATACACAGAGGACCGCCTGGGGCTAAAGAAAATGGACAAAGCAGGAAAGCTA
GTGTTTCTGGCTAAGGAAGGGGACCATCTTCAAATATCTAAAGAATGGTTTACTGCCCACATCATACCTT
TTCTTAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008917
Insert Size 921 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008917.2, NP_032943.2
RefSeq Size 2478 bp
RefSeq ORF 921 bp
Locus ID 19063
UniProt ID O88531
Cytogenetics 4 57.27 cM
Gene Summary Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.